摘要
建立了毛细管气相色谱法检测桑叶中农药多残留的方法。样品以乙腈为提取剂,提取液用弗罗里硅土柱净化,乙酸乙酯∶正己烷(体积比15∶85)洗脱,采用HP-5与DB1701毛细管色谱柱双柱,气相色谱-电子捕获检测法(GC-ECD)测定42种有机氯及拟除虫菊酯类农药及其异构体的残留量。结果表明:该方法在20、80、160μg/kg 3个添加水平下回收率分别为78.6%～110.3%、78.3%～109.6%、80.1%～109.1%,相对标准偏差(RSD)均在1.1%～11.3%,符合农药残留分析的要求。该方法重复性及净化效果好,可用于桑叶中42种有机氯及拟除虫菊酯类农药及其异构体的残留量检测。
A gas chromatography method for the determination of pesticides multi-residues in mulberry leaf was established.The organochlorine and pyrethroid pesticides were extracted from mulberry leaf with petroleum acetonitrile.Then the extracts were cleaned up by alumina neutral-florisil column and eluted by mixed solvents of ethyl acetate and hexane（15∶85,V/V）.The extracts were separated by HP-5 and DB1701dual capillary column and detected by electron-capture detector.Results show that the recoveries were from 78.6%to 110.3%,from 78.3%to 109.6%and from 80.1%to 109.1% with RSD ranged from 1.1%to 13.1%,at three spiked mixed organochlorine and pyrethroid levels,respectively.The method has good separation ability and repeatability and can be used in determination of organochlorine and pyrethroid pesticide residues in Mulberryleaf products.
出处
《分析科学学报》
CAS
CSCD
北大核心
2012年第3期328-332,共5页
Journal of Analytical Science
基金
国家重大创新药专项(No.2009ZX09502-027
2009ZX09308-006
2009ZX09308-001-1-5)
关键词
桑叶
毛细管气相色谱
有机氯农药
拟除虫菊酯农药
Mulberryleaf
Capillary gas chromatography
Organochlorine pesticide
Pyrethroid pesticide
