摘要
采用平板分离法和柠檬酸铁还原实验法相结合,从城市污水处理厂活性污泥中分离获得Fe(Ⅲ)还原菌F7,经形态观察、生理生化和16S rDNA序列分析及同源性比对鉴定为恶臭假单胞菌(Pseudomonas putida)。在不同柠檬酸铁浓度和不同pH条件下的实验表明,柠檬酸铁浓度为0.32 g/L时,菌株生长情况较好,柠檬酸铁浓度为0.16 g/L时,Fe(Ⅲ)异化还原比例较高;pH 6.5时,菌株生长情况较好,Fe(Ⅲ)异化还原量较多。
A ferric reducing microorganism strain F7 was isolated from activated sludge taken from wastewater treatment plant through dilution-plate method and ferric citrate reduction. By means of the morphology, physiology and the 16S rD- NA sequence analysis and homology comparison, strain F7 was identified as Pseudomonas putida. Different ferric citrate fluid medium concentration and different pH experiments showed that strain F7 growed well with O. 32 g/L ferric citrate, and Fe（Ⅲ） reduction ratio was high with 0.16 g/L ferric citrate,and the bacteria growth and Fe（ Ⅲ ） reduction quanti- ty were well with pH 6.5.
出处
《天然产物研究与开发》
CAS
CSCD
北大核心
2012年第5期627-630,643,共5页
Natural Product Research and Development
基金
国家"十一五"科技重大专项--三峡库区城市污水处理厂功能提升与污泥处理处置技术研究与综合示范(2009ZX07315-002)
