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沙眼衣原体通过激活MAPK/ERK信号通路抑制宿主细胞凋亡 被引量：2

Chlamydia trachomatis Inhibit Apoptosis of Host Cells by Activation of the MAPK/ERK Signaling Pathway
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摘要 目的:研究沙眼衣原体抑制宿主细胞凋亡活性与MAPK/ERK信号通路的关系。方法:利用化学抑制剂U0126阻断MAPK/ERK信号通路,然后分别采用流式细胞术、Caspase-3活性检测试剂盒和Western Blot实验检测沙眼衣原体感染细胞在凋亡诱导剂Etoposide作用下细胞凋亡率和Caspase-3活性变化,以及PARP是否发生裂解。结果:当MAPK/ERK信号通路被阻断时,在Etoposide的作用下,沙眼衣原体感染细胞凋亡率明显上升,同时Caspase-3被活化和PARP发生裂解。结论:沙眼衣原体抑制宿主细胞凋亡活性与MAPK/ERK信号通路激活有关。 Objective： To investigate the relation between inhibition of apoptosis and MAPK/ERK signaling pathway in Chlamydia trachomatis-infected cells. Methods： MAPK/ERK signaling pathway was blocked by U0126 in Chlamydia trachomatis-infected cells, then the percentage of apoptotie cells, Capase-3 activity and PARP cleavage, which were induced by etoposide, were detected with flow cytometer, caspase-3 activity kit and Western Blot assay, respectively. Re- suits：The percentage of apoptotic cells induced by etoposide was significantly higher, Caspase-3 was activated and PARP was cleft too, when MAPK/ERK signaling pathway was blocked by U0126. Conclusion：Chlamydia trachomatis inhibit o apoptosis of host cells involves activation of the MAPK/ERK signaling pathway.
作者 杜昆 朱丽莎 艾彪 刘天春
机构地区 湖北省荆州市第一人民医院检验科
出处 《激光生物学报》 CAS CSCD 2012年第2期141-144,共4页 Acta Laser Biology Sinica
关键词 沙眼衣原体 MAPK/ERK 细胞凋亡 Chlamydia trachomatis .MAPK/ERK apoptosis
分类号 R374.1 [医药卫生—病原生物学]
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参考文献13

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二级参考文献17

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共引文献5

同被引文献23

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  • 5Kyriakis JM, Avruch J. Sounding the alarm: protein kinase cascades activated by stress and inflammation [ J]. J Biol Chem, 1996, 271 (40) :24313 -24316.
  • 6Kaplan DR, Miller FD. Neurotrophin signal transduction in the nervous system[J]. Curr Pin Neurobiol, 2000, 10(3) :381 -391.
  • 7Yan CY, Greene LA. Prevention of PC12 cell death by N-acetylcysteine requires activation of the Ras pathway[ J]. J Neurosci, 1998, 18 ( 11 ) : 4042 - 4049.
  • 8Tran SEF, Holmstrom TH, Ahonen M, et al. Mitogen-activated protein kinase/ERK overrides the apoptotic signaling from Fas, tumor necrosis factor alpha, and TRAIL receptors[ J]. J Biol Chem, 2001,276(19) : 16484 - 16490.
  • 9Fan T, Lu H, Hu H, et al. Inhibition of apoptosis in Chlamydia-infec- ted cells : blockade of mitochondrial cytochrome release and caspase ac- tivation[J]. J Exp Med, 1998, 187(4) : 487 -496.
  • 10Su H, McClarty G, Dong F, et al. Activation of Raf/MEK/ERK/cP LA2 signaling pathway is essential for Chlamydial acquisition of host glycerophospholipids [ J]. J Biol Chem, 2004, 279 (10): 9409 - 9416.

引证文献2

二级引证文献4

激光生物学报
2012年 第2期

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