摘要
选择与羊同源性较高的牛RERG基因组序列设计特异性引物,提取黔北麻羊脾脏总RNA,通过RT-PCR技术对RERG基因进行克隆测序及序列分析。结果表明:首次克隆了黔北麻羊RERG基因cDNA序列629 bp,GenBank登录号为JN672576,该基因共编码199个氨基酸。黔北麻羊RERG基因与牛的RERG基因同源性高达98.5%。聚类分析显示:哺乳动物、禽类和两栖类各为一类。该聚类结果与动物间遗传距离大小一致,也与各动物在动物学上的分类相吻合,说明RERG基因编码区适于构建种间系统进化树。
Selection of cow RERG genome sequence which is higher homology with sheep.A specific primers had been designed.Total RNA was extracted from the Qian Bei ma goat of spleen and the cDNA encoding RERG was obtained by the reverse transcription PCR（RT-PCR）.The purified RT-PCR product was cloned into T vector,and then the sequence was analyzed.The results demonstrated that the 629 bp product was the Qian Bei ma goat RERG cDNA,and GenBank accession number was JN672576 respectively.It encoded 199 amino acids.RERG gene in Qian Bei ma goat had 98.5% homology with cow.Cluster analysis revealed that probably mammals,birds and amphibians,each of these belonged to different categories separately.This result of phylogenetic clustering was identical to the genetic distance and zoological classification,which indicated that the RERG gene was also fit to construct molecular phylogenetic tree among different species.
出处
《广东农业科学》
CAS
CSCD
北大核心
2012年第8期148-151,共4页
Guangdong Agricultural Sciences
基金
贵州大学研究生创新基因(农科2012036)
