摘要
[目的]探讨富组蛋白对表皮创面愈合的影响,为临床用药和创面护理提供新的思路和方向。[方法]通过细胞划痕试验,分为Hist1实验组、空白对照组、Hist1+丝裂霉素实验组、空白+丝裂霉素对照组,用丝裂霉素抑制细胞增殖,建立体外创面模型,于0h、16h、24h分别测量划痕区1.4 mm2内的愈合率。[结果]大约1.4mm2的创面16h愈合率为64.60%,24h愈合率为71.42%;而空白对照组16h愈合率为41.23%,24h愈合率为48.11%,两组相比,差异有统计学意义(P<0.05);而加入丝裂霉素后,16h愈合率为61.97%,明显高于空白+丝裂霉素对照组(32.25%)。[结论]30μg/mL生理浓度的富组蛋白1能明显促进表皮细胞增殖和迁移,且随着时间延长愈合率增高;提示富组蛋白1能促进非口腔上皮创面愈合。
Objective：To probe into the influence of histatins 1 on epidermal wound healing,so as to provide the new thinking and directions for clinical medication and wound care. Methods：The cases were divided into Hist 1 group, blank control group, Histl 4- mitomyein experimental group,blank 4- mitomycin control group through cell scratch test, using mitomycin to inhibit cell proliferation and establish in vitro wound model and measure the healing rate in 1 mm scratch area respectively at the 0 h, 16 h,24 h. Results：The healing rate of 1.4 mm2 wound was 64.60% after 16 h,71.42% after 24 h;while the healing rate of blank control group was 41.23% after 16 h,was 48. 11% after 24 h,and the difference was statistically significant between two groups （P〈 0. 05） ; however, after adding mitomycin, healing rate was 6l. 97 % after 16h,it was significantly higher than that of blank group （32. 25%）. Conclusion：30 μg/mL physiological concentrations of histatins 1 can obviously promote epidermal cell proliferation and migration, and the healing rate increased with the prolongation of time,it prompted the histatinl can promote the nonoral epithelial wound healing.
出处
《护理研究（上旬版）》
2012年第5期1166-1167,共2页
Chinese Nursing Researsh
关键词
创面
富组蛋白1
愈合
实验
wound
histatins 1
healing
experiment
