摘要
【目的】探讨鸡血藤与其常见混淆品的薄层色谱鉴别条件,并开展鸡血藤薄层色谱指纹图谱的初步研究,分析不同居群鸡血藤野生种质在化学组分上的差异。【方法】采用三氯甲烷—甲醇—甲酸(体积比为7∶1∶0.2)和三氯甲烷—丙酮—甲酸(体积比为8∶1∶0.5)为展开剂,体积分数5%硫酸乙醇溶液显色,可鉴别鸡血藤与其常见混淆品;三氯甲烷—甲醇—甲酸(体积比为10∶1∶0.4)能很好地分离不同居群鸡血藤中的组分,采用Ntsys 2.10软件非加权组平均法(UPGMA)聚类分析不同居群鸡血藤种质品质间的差异性。【结果】建立了鸡血藤与其常见混淆品的薄层色谱鉴别方法;建立了13个特征荧光斑点构成的鸡血藤薄层色谱指纹图谱。通过聚类分析,可将11个不同居群正品鸡血藤归为4类。【结论】薄层色谱法可以有效地鉴别鸡血藤类药材,不同居群鸡血藤野生种质品质间存在较大的差异性。
Objective To investigate thin layer chromatography（TLC）conditions for the identification of Caulis Spatholobi and its common adulterants,and to study the differences in chemical composition of different populations of wild germplasm through TLC fingerprint analysis.Methods With the mixture of chloroform-methanol-formic acid（volume ratio being 7 ∶ 1 ∶ 0.2） and chloroform-acetone-formic acid（volume ratio being 8 ∶ 1 ∶ 0.5） as developing agents,and with 5%（volume fraction） sulfuric acid-ethanol solution as the color developing reagent,TLC was used for the identification of Caulis Spatholobi and its common adulterants.The composition of different populations of Caulis Spatholobi was well separated with the mixture of chloroform-methanol-formic acid（volume ratio being 10 ∶ 1∶ 0.4）.Finally,we used unweighted pair-group method of Ntsys 2.10 software and arithmetic means（UPGMA） for cluster analysis of the quality differences in various populations of Caulis Spatholobi.Results We have established TLC method for the identification of Caulis Spatholobi and its common adulterants,and established Caulis Spatholobi TLC fingerprints which consisted of 13 specific fluorescent spots.Through cluster analysis,11 populations of genuine Caulis Spatholobi were grouped into four categories.Conclusion TLC can be used to effectively identify the medicinal material of Caulis Spatholobi.Great variability exists in different populations of wild germplasm of Caulis Spatholobi.
出处
《广州中医药大学学报》
CAS
北大核心
2012年第3期305-307,340,共4页
Journal of Guangzhou University of Traditional Chinese Medicine
基金
广东省重大科技专项(编号:2007A032200001)
广东省教育部科技部企业科技特派员行动计划专项(编号:2009B090600135)
关键词
鸡血藤/化学
色谱法
薄层
CAULIS SPATHOLOBI/chemistry
CHROMATOGRAPHY
THIN LAYER
