摘要
目的分析老年住院患者感染产金属酶的铜绿假单胞菌耐药现状,指导临床合理使用抗菌药物。方法采用法国生物梅里埃公司的API微生物自动鉴定系统,对医院2008-2010年,老年病房住院患者的各类临床标本进行细菌分离,药敏试验采用纸片扩散法进行检测;用双纸片协同法检测铜绿假单胞菌产金属酶。结果分离的1056株铜绿假单胞菌中耐亚胺培南的菌株有205株,检出率为19.4%,其中产金属酶的有57株,阳性率为27.8%;耐亚胺培南铜绿假单胞菌主要分布于老年重症病房、老年呼吸内科、老年神经内科,耐亚胺培南铜绿假单胞菌与亚胺培南敏感铜绿假单胞菌耐药性有明显差异。结论加强金属β-内酰胺酶检测,按照药敏试验选择治疗药物,以防止菌株扩散是非常重要的。
OBJECTIVE To explore the current status of drug resistance of metallo-13-1actamase（MBL）-producing in clinical isolates of Pseudomonas aeruginosa in senile patients so as to guide the clinical reasonable use of antibiotics. METHODS The clinical samples were collected from elderly patients in geriatric wards from 2008 to 2010 were identified and analyzed by VPI system, the drug susceptibility testing was performed by disk diffusion, and double disc synergy test was used to detect P. aeruginosa producing metallo-13-1actamase. RESULTS Of 1056 strains of P. aeruginosa isolated, there were 205 strains of im^penem-resistant P. aeruginosa with the detection rate of 19.4% and 57 strains of metallo-β-1actamase-producing P. aeruginosa with the positiverate of 27.8% IRPA mainly distributed in the senile ICU, senile respiratory medicine department, and senile neurology department; there was significant difference in drug resistance between IRPA and imipenem-susceptible P. aerug- inosa （ISPA）. CONCLUSION It is necessary to strengthen the detection of MBL and use antibiotics on the basis of drug susceptibility testing so as to prevent the prevalence of the isolates.
出处
《中华医院感染学杂志》
CAS
CSCD
北大核心
2012年第11期2434-2436,共3页
Chinese Journal of Nosocomiology
基金
湖南省医药卫生科研计划项目(B2009069)
