摘要
目的调查河南省信阳市虫媒病毒的种类。方法 2009年8月在河南省信阳市息县采集蚊虫标本,利用BHK-21细胞培养分离虫媒病毒,对能够引起稳定的细胞病变的分离株利用虫媒病毒特异引物进行RT-PCR鉴定,RT-PCR结果阳性者进行序列测定。结果共采集蚊虫标本3 050只(36批),3批标本(XY0901、XY0904和XY0927)在BHK-21细胞中出现显著的、稳定的病变;2株病毒(XY0901/ChinaHN株和XY0904/China HN株)以甲病毒属特异引物进行RT-PCR检测,结果为阳性,XY0927/ChinaHN株以流行性乙型脑炎(乙脑)病毒特异引物、各虫媒病毒属特异引物进行RT-PCR检测,结果均为阴性;XY0901/China HN株和XY0904/China HN株测序结果比对与盖塔病毒核苷酸同源性为98.0%～99.1%。结论 2009年在河南省分离到2株盖塔病毒和1株未知虫媒病毒。盖塔病毒为河南省首次分离。
Objective To isolate and identify arboviruses from mosquito pools in Xinyang city of Henan province in 2009.MethodsMosquitoes were collected from Xinyang city of Henan province in 2009.Isolated viruses were inoculated into BHK-21 cells and identified the virus species by PCR and sequencing.ResultsA total of 3 050 mosquitoes were collected and three isolates produced CPE in BHK-21 cell culture with two isolates were identified as Getah virus and one unknown strain,respectively.ConclusionsTwo strains of Getah virus and one unknown strain were isolated from mosquitoes and reported first time in Xinyang city of Henan province.
出处
《中国病毒病杂志》
CAS
2012年第3期207-210,共4页
Chinese Journal of Viral Diseases
基金
河南省医学科技攻关项目(200702016)
