摘要
目的:探讨"双固一通"配穴电针对老年阳虚模型大鼠学习记忆改善作用与免疫衰老调控机制。方法:选用5月龄雌性SD大鼠40只,随机分为正常对照组、老年阳虚模型组、双固一通电针组、电针对照组,每组10只。除正常对照组外,其余3组大鼠皮下注射D-半乳糖40d后再肌肉注射氢化可的松7d制作老年阳虚模型。双固一通电针组取"关元""后三里""百会"治疗,电针对照组取"中极""阴陵泉""印堂"治疗,均每周治疗6次,共治疗4周结束。电针治疗第4周第1天开始进行Morris水迷宫试验,检测大鼠逃避潜伏期;电针治疗结束后用流式细胞术检测大鼠脾脏淋巴细胞凋亡率;酶联免疫吸附法检测大鼠血清肿瘤坏死因子(TNF-α)含量。比较各组之间的差异。结果:与正常对照组比较,老年阳虚模型组大鼠逃避潜伏期[(25.4±3.6)s vs(16.23±2.3)s]、脾脏淋巴细胞凋亡率[(27.25±3.3)%vs(13.2±3.1)%]和血清TNF-α含量[(15.54±3.56)pg/mL vs(7.35±2.89)pg/mL]均增高(均P<0.01);与老年阳虚模型组相比,双固一通电针组逃避潜伏期(17.42±3.9)s、脾脏淋巴细胞凋亡率(17.2±3.25)%和血清TNF-α含量(9.51±3.53)pg/mL,均明显降低(均P<0.01),而与电针对照组比较,上述各指标双固一通电针组亦降低明显(均P<0.05)。结论:"双固一通"配穴电针治疗老年阳虚模型大鼠能明显改善空间学习记忆能力,其作用机制与电针能降低老年阳虚模型大鼠脾脏淋巴细胞凋亡率和血清中TNF-α的表达有关,且"双固一通"配穴电针"关元""后三里""百会"的作用效应优于电针"中极""阴陵泉""印堂"配穴治疗效果。
Objective To explore the improvement effect and the regulating mechanism of electroacupuncture with ＂Shuanggu Yitong＂ prescription methods on learning memory and immunosenescence in aging rats with yang deficiency.Methods Forty female Sprague-Dawley rats,5-month old,were randomly divided into a normal control（NC） group,an aging yang deficiency model（M） group,an electroacupuncture with Shuanggu Yitong prescription methods（EA） group and an electroacupuncture control（EAC） group,10 rats in each group.The aging model rats with yang deficiency were established by hypodermic injection of D-galactose for 40 days and then intramuscular injection of Hydrocortisone for 7 days in the latter three groups except the NC group.The EA group was treated with electroacupuncture at ＂Guanyuan＂（CV 4）,＂Housanli＂（ST 36） and ＂Baihui＂（GV 20）,and the EAC group was treated with electroacupuncture at ＂Zhongji＂（CV 3）,＂Yinlingquan＂（SP 9） and ＂Yintang＂（EX-HN 3）,six times per week for 4 weeks.The escape latency was examined by Morris water maze from the first day of the 4th week.After completion of electroacupuncture treatment,the rate of spleen lymphocyte apoptosis and the serum content of tumor necrosis factor-α（TNF-α） were examined by flow cytometry and enzyme linked immunosorbent assay（ELISA） technique respectively,the differences among all the groups above were compared.Results In comparison with the NC group,the escape latency [（25.4±3.6）s vs（16.23±2.3）s],the rate of spleen lymphocyte apoptosis [（27.25±3.3） %vs（13.2±3.1） % ] and the serum content of TNF-α [（15.54±3.56） pg / mL vs（7.35±2.89） pg / mL ] in the M group those were all significantly increased（all P0.01）.In the EA group,the escape latency of（17.42±3.9） s,the rate of spleen lymphocyte apoptosis of（17.2±3.25） %and the serum content of TNF-αof（9.51±3.53） pg / mL were all significantly lower than those in the M group（all P0.01） and the EAC group（all P0.05）.Conclusion Electroacupuncture with ＂ Shuanggu Yitong ＂ prescription can significantly improve the spatial learning and memory.The protective mechanism is related with the action of electroacupuncture in lowering the rate of spleen lymphocyte apoptosis and the serum content of TNF-α,and the therapeutic effect of electroacupuncture at ＂ Guanyuan ＂（CV 4）,＂ Housanli ＂（ST 36） and ＂ Baihui ＂（GV 20） is superior to that of electroacupuncture at ＂ Zhongji ＂（CV 3）,＂ Yinlingquan ＂（SP 9） and ＂ Yintang ＂（EX-HN 3）.
出处
《中国针灸》
CAS
CSCD
北大核心
2012年第6期529-533,共5页
Chinese Acupuncture & Moxibustion
基金
湖北省自然科学基金重点项目:2008CDA051
关键词
衰老大鼠
阳虚证
电针
“双固一通”配穴
细胞凋亡
肿瘤坏死因子-Α
Aging Rat
Yang Deficiency
Electroacupuncture
Shuanggu Yitong Point
Spleen Cell Apoptosis
Tumor Necrosis Factor-α
