摘要
目的探讨肿瘤坏死因子(TNF)-β+252基因多态性分布及人乳头瘤病毒(HPV)感染与我国子宫颈癌高发区新疆喀什维吾尔族子宫颈癌易感性的关系。方法以导流杂交基因芯片技术检测111例子宫颈癌和100例正常子宫颈组织HPV感染及型别分布;用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)方法对83例HPV16阳性子宫颈癌及91例HPV阴性正常子宫颈组织检测肿瘤坏死因子-β+252位点的基因型,分析肿瘤坏死因子-β+252基因多态性及HPV感染与维吾尔族子宫颈癌遗传易感性的关系。结果 111例子宫颈癌患者中,HPV总感染率81.1%(90/111),其中HPV16感染占HPV阳性子宫颈癌的92.2%(83/90),100例正常子宫颈组织中HPV阳性者9例,阳性率为9.0%(9/100),其中HPV16感染2例,两组之间差异有统计学意义(χ2=115.8,P<0.01)。TNF-β+252位点基因多态性与HPV16阳性子宫颈癌组及HPV阴性对照组之间差异无统计学意义(P>0.05)。结论新疆南部维吾尔族妇女子宫颈癌患者中以HPV16感染为主,本研究未能显示肿瘤坏死因子-β(+252)位点多态性与维吾尔族子宫颈癌易感性有关。
Objective To investigate the association of polymorphism distribution of HPV infection and tumor necrosis factor TNF-β（＋252） genes with the susceptibility to cervical cancer of Uighur Women from the Southern Xinjiang the high risk region of cervical cancer.Methods HPV was detected by flow-through hybridization and gene chip（HybriMax） method in 111 cases of cancer tissues and 100 of normal cervical samples,TNF-β（＋252） genotypes were genotyped by PCR-RFLP in 83 cases of HPV16-positive cervical cancer tissues and 91 of HPV-negative normal cervical tissues.Results The positive rate of HPV in cervical cancer and control group was 81.1%（90/111） and 9.0%（9/100）,respectively;HPV16 was the most common type in HPV positive cervical cancer patients with the rate of 92.2 %（83/90）,in control group only 2 cases showed HPV16 infection.The positive rate of HPV16 in cervical cancer group was significantly higher than that in control group（χ2 =115.8,P0.01）.There were no significant differences in the frequencies of genotype of TNF-β（＋252） locus between HPV16-positive cervical cancer and HPV negative control group.Conclusions HPV16 is the most common type in cervical cancer of Uighur women.Association is not found between the polymorphism of TNF-β（＋252） locus and the susceptibility to cervical carcinoma of Uighur women.
出处
《实用肿瘤杂志》
CAS
2012年第3期251-254,共4页
Journal of Practical Oncology
