摘要
目的探讨姜黄素对体外培养大鼠肝星状细胞(HSC)增殖、转化生长因子β1(TGF-β1)及其受体、下游信号通路及效应的影响。方法培养HSC细胞,给予不同浓度姜黄素处理,采用MTT比色法检测HSC细胞增殖;Hoechst 33342染色检测姜黄素对HSC凋亡的影响;采用免疫细胞化学法检测Smad3、Smad7蛋白表达;用逆转录聚合酶链式反应(RT-PCR)检测TGF-β1及其I、II受体(TβRI、TβRII)和I、III型胶原(Col-I、Col-III)mRNA的表达。结果姜黄素能抑制HSC细胞增殖,诱导HSC细胞凋亡,免疫组化结果显示姜黄素能抑制HSC表达Smad3,并提高Smad7表达,RT-PCR结果显示姜黄素使HSC细胞TGF-β1及其I、II受体、Col-I、Col-III mRNA表达降低。结论姜黄素能抑制HSC增殖和活化,抑制TGF-β1及其受体和信号通路,是其抗肝纤维化的作用机制之一。
Objective To observe the inhibition effect of curcumin on hepatic stellate cells（HSC） cell proliferation,mRNA expression of transforming growth factor-beta1（TGF-β1） and its receptors,and signal transduction.Methods The proliferation of HSC was examined by MTT colorimetric.The apoptosis of HSC was examined by Hoechst 33342;The mRNA levels of TGF-β1,TβRI,TβRII,collagen I（Col-I）,and collagen III（Col-III） were examined by RT-PCR;The expression of Smad3 and Smad7 in HSC was examined by immuneohistochemical method.Results Curcumin could inhibit HSC proliferation and induce HSC apoptosis;What’s more,the mRNA levels of TGF-β1,TβRI,TβRII,Col-I,and Col-III,and the expression of Smad3 was significantly decreased by curcumin;The expression of Smad7 was remarkably increased.Conclusion Curcumin could inhibit the proliferation of HSC,inhibit the expression of TGF-β1,and its receptors as well as its signal transduction,which could result in the effect of antihepatic fibrosis.
出处
《中草药》
CAS
CSCD
北大核心
2012年第5期957-961,共5页
Chinese Traditional and Herbal Drugs
基金
广东省中医药局项目(2009276)
广东食品药品职业学院院级项目(2007012)
