摘要
目的:检测小干扰RNA对慢性粒细胞白血病细胞系K562的影响。方法:脂质体转染细胞,MTT实验检测转染后的细胞活性,并绘制生长曲线,用Western blot检测p210蛋白表达水平的变化,并用Annexin-V-FITC法检测细胞凋亡水平。结果:与对照组相比,有2条小干扰RNA序列可以有效地降低细胞活性,诱导细胞凋亡并降低p210蛋白的表达水平。结论:小干扰RNA可抑制K562细胞活性,降低融合蛋白表达水平并诱导细胞,有望成为治疗慢性粒细胞白血病的有效方法。
Objective： To detect the influention of small interfering RNA on the chronic myelogenous leukemia cell line K562.Methods：Transfected the cells with liposome,and tested the cell activation with MTT assay,then drew the growth curve.Measured the expression level of p210 protein and tested the cell apoptosis with Annexin-V-FITC.Results：Compared with the negative control,two siRNAs could reduce the cell activation,induce cell apoptosis and reduce the expression of p210.Conclusion：Small interfering RNA could inhibit cell activation of K562,reduce the expression of fusion protein and induce the apoptosis;which might be a valid therapy for chronic myelogenous leukemia.
出处
《海南医学院学报》
CAS
2012年第6期727-730,共4页
Journal of Hainan Medical University
基金
海南大学"211工程"三期建设研究生科技创新平台(创新实验室)~~
