摘要
目的:观察大鼠全脑缺血模型海马细胞凋亡情况与RTP801mRNA的表达,探讨RTP801对全脑缺血的作用及其机制。方法:36只大鼠随机分为实验组(30只)和对照组(6只),实验组采Pulsineli用4血管阻断法制做大鼠全脑缺血模型,并按照缺血后6h、12h、24h、48h、72h随机分为5个小组,每组6只大鼠。在各个时间点处死大鼠,应用TUNEL染色观察大脑海马神经元凋亡情况,采用RT-PCR技术检测大鼠海马部RTP801mRNA表达水平。结果:全脑缺血组后各个时间点神经元凋亡差异有统计学意义(P<0.05),其中缺血48h神经元凋亡最明显。RT-PCR结果显示,RTP801mRNA表达水平在缺血后6h开始明显增加,12h达高峰;除72h组外,RTP801mRNA表达水平与对照组相比差异有统计学意义(P<0.05)。结论:细胞凋亡在全脑缺血大鼠模型缺血损伤过程中起重要作用,全脑缺血时大鼠海马RTP801表达明显增高可能加重凋亡所致的神经损伤,其具体机制尚不完全清楚。
Objective： To explore the Hippocampal apoptosis and RTP801mRNA expression in global brain ischemia model of rats,and to discuss the role of RTP801 in the mechanism of global brain ischemia.Methods： A total of 36 cases of rats were randomly divided into experimental group（30 rats） and control group（6 rats）.Four vessel occlusion method was used to establish rat models of global cerebral ischemia.After establishment of ischemia,rat models were subdivided into five groups with 6 rats in each group according to time of ischemia,viz.6 h after ischemia,12 h after ischemia,24 h after ischemia,48 h after ischemia.Rats were sacrificed at the above mentioned time points,TUNEL staining method were used to observe hippocampal neuronal apoptosis,RT-PCR detection was applied to evaluate RTP801mRNA.expression level.Results： Ischemia groups at each time point showed significant difference in neuronal apoptosis（P0.05）,of which neuron apoptosis was most significant in group of 48 h ischemia.RT-PCR results showed that RTP801mRNA expression level significantly increased at 6 h after ischemia,and reached the peak at 12 h;Except 72 h ischemia group,RTP801mRNA expression levels were significantly differed from that of the control group（P0.05）.Conclusions： Apoptosis play an important role in the process of injury occurred in global ischemia rat model.The elevated hippocampus RTP801 expression level may induce neuronal injury caused by apoptosis.
出处
《海南医学院学报》
CAS
2012年第6期724-726,共3页
Journal of Hainan Medical University
基金
中国高校医学期刊临床专项资金项目(112210166)~~
