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用于组织工程骨的磁性聚乳酸乙醇酸共聚物-骨形态发生蛋白2微球的合成及检测

Synthesis and detection of magnetic poly lactic acid-glycolic acid copolymer-bone morphogenetic protein 2 microspheres applied in tissue engineered bone
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摘要 背景：组织工程骨支架常因支架材料的孔隙率不佳和无法以合适的方式提供生物活性因子给种子细胞,导致支架对细胞的黏附及诱导性不强,成骨效率低下。目的：合成一种新型的可用于组织工程骨的磁性聚乳酸乙醇酸共聚物-骨形态发生蛋白2缓释微球并对其各项性能进行检测分析。方法：复乳法合成磁性聚乳酸乙醇酸共聚物-骨形态发生蛋白2微球。结果与结论：扫描电镜下可见微球呈正圆形,粒径10~100μm;激光共聚焦显微镜下可见重组人骨形态发生蛋白2在微球上分布均匀;振动样品磁强计检测结果显示微球具有超顺磁性;微球载药量为（1.00±0.18）μg/g;微球对其具有缓释效果;经45℃处理微球后测得微球载药量为（0.98±0.20）μg/g。说明合成的磁性聚乳酸乙醇酸共聚物-骨形态发生蛋白2缓释微球具有一系列良好的特性,为新型磁性组织工程骨支架的研制奠定了基础。 Tian Zhi-chao1, Yang Yang2, Xiao Jun1, Qiu Jin-jun3, Zhu Yuan-li4, Li Liang-yu1 1Department of Orthopedics, 4Department of Pathology, Tongji Hospital, Tongji Medical College of Huazhong University of Science and Technology, Wuhan 430030, Hubei Province, China; 2Department of Information Engineering, Zhengzhou Huaxin College, Zhengzhou 451100, Henan Province, China; 3School of Chemistry and Chemical Engineering, Huazhong University of Science and Technology, Wuhan 430074, Hubei Province, China
作者 田志超 杨杨 肖骏 邱进俊 朱元莉 李亮宇
机构地区 华中科技大学同济医学院附属同济医院骨科 郑州华信学院信息工程系 华中科技大学化学与化工学院 华中科技大学同济医学院附属同济医院病理科
出处 《中国组织工程研究》 CAS CSCD 2012年第16期2927-2930,共4页 Chinese Journal of Tissue Engineering Research
基金 武汉市科技攻关计划资助项目(200960223069)~~
关键词 磁性微球 聚乳酸-乙醇酸共聚物 骨形态发生蛋白2 组织工程支架 骨
分类号 R318 [医药卫生—生物医学工程]
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参考文献11

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二级参考文献9

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中国组织工程研究
2012年 第16期

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