摘要
目的:检测结直肠癌组织中Bmi-1、PTEN和E-Cadherin的表达,探讨三者表达的相关性及意义。方法:用Western blot检测Bmi-1在5对结直肠癌和癌旁活检组织中的表达,实时定量PCR法检测Bmi-1、PTEN和E-Cadherin mRNA在28对结直肠癌和癌旁活检组织的表达;用免疫组织化学法检测Bmi-1、PTEN和E-Cadherin在28例结直肠癌石蜡组织中的表达及其相关性。结果:Bmi-1蛋白在5例结直肠癌活检组织中的表达明显高于癌旁组织;Bmi-1 mRNA在893%(25/28)的癌组织中的表达高于癌旁组织;PTEN和E-CadherinmRNA在82.1%(23/28)的癌组织中的表达低于癌旁组织;统计学分析发现Bmi-1和PTEN及E-Cad-herin的mRNA表达水平呈负相关;28例结直肠石蜡组织中,Bmi-1、PTEN和E-Cadherin蛋白的阳性率分别为92.9%(26/28)、46.4%(13/28)和82.1%(23/28),统计学分析发现Bmi-1和PTEN及E-Cadherin蛋白表达水平呈负相关。结论:本研究发现Bmi-1和PTEN及E-Cadherin之间呈负相关,从组织水平证实Bmi-1对PTEN及E-Cadherin的调控关系,为Bmi-1作为预测结直肠癌发生及转移的分子标志物及治疗新靶点提供了新的理论依据。
Objective： To determine the expression of fBmi-1, PTEN, and E-cadherin in colorectal cancer, and analyze the correlation among Bmi-1, PTEN and E-cadherin. Methods： Western blot analysis was performed to detect Bmi-1 expression in five colorectal cancer biopsies and matched adjacent noncancerous tissues. Real-time reverse transcription polymerase chain reaction （ RT-PCR ） was performed to determine the protein and mRNA levels of Bmi-1, PTEN, and E-cadherin in 28 colorectal cancer biopsies and matched adjacent non-cancerous tissues. The protein levels of Bmi-1, PTEN, and E-cadherin in 28 paraffin-embedded colorectal cancer tissues were detected via immunohistochemistry. Statistical analysis was performed to evaluate the association among Bmi-1, PTEN, and E-cadherin at both the mRNA level and the protein level. Results： Bmi-1 was clearly upregulated in the 5 colorectal cancer biop- sies and their matched adjacent non-cancerous tissues. Bmi-1 mRNA was upregulated in 89.3% （ 25/28 ） of colorectal cancer biopsies compared with the adjacent noncancerous tissues. PTEN and E-cadherin mRNA were downregulated in 82.1% （ 23/28 ） of colorectal cancer biopsies compared with adjacent noncancerous tissues. High Bmi-1 mRNA expression was significantly correlated with low lev- els of PTEN and E-cadherin mRNA. Immunohistochemistry and statistical analyses revealed that high Bmi-1 protein expression was significantly associated with low levels of PTEN and E-cadherin. Conclusion： This study provided evidence of the negative regulation of PTEN and E-cadherin by Bmi-1 at the clinical tissue level. Bmi-1 is a biomarker for colorectal cancer progression and metastasis and may be used as a therapeutic target.
出处
《中国肿瘤临床》
CAS
CSCD
北大核心
2012年第9期559-563,共5页
Chinese Journal of Clinical Oncology
基金
国家自然科学基金(编号:30901791
81071735)资助~~
