摘要
目的建立芡实药材指纹图谱,并进行主成分分析和聚类判别。方法芡实的70%甲醇萃取液采用高效液相色谱法,以Hypersil ODS柱(4.6 mm×200 mm,5μm)为色谱柱,乙腈-1%乙酸水溶液进行梯度洗脱,体积流量1.0 mL/min,检测波长273 nm,柱温30℃。结果建立了14个产地药材的共有图谱,确定了17个共有峰,得到3个决定峰,聚成五类,并比较了样品中柚皮素的含有量。结论主成分分析结合聚类判别所建立的HPLC指纹图谱及定量分析方法均具有快速稳定的特点。
AIM To establish an HPLC fingerprint of Euryales Semen collected from fourteen habitats,and to make evaluation by principal component analysis（PCA） and cluster analysis（CA）.METHODS HPLC analysis of 70% methanol extract of Euryales Semen was carried out by Hypersil ODS column（4.6 mm ×200 mm,5 μm） with mobile phase of acetonitrile-1% acetic acid system in gradient elution mode with a flow at 1.0 mL/min,an ultraviolet detection wavelength at 273 nm for fingerprint and column temperature at 30 ℃.RESULTS Seventeen common peaks were identified in chromatograms with reference to naringenin peak from the 14 batches of the samples.Three main peaks were obtained and all the peaks were classified into five categories.CONCLUSIONThe method of the HPLC fingerprint and quantitative analysis established by PCA-CA can be used for the classification of Euryales Semen from different habitats and has the characteristic of rapidness and stability.
出处
《中成药》
CAS
CSCD
北大核心
2012年第5期781-787,共7页
Chinese Traditional Patent Medicine
基金
国家药典委员会项目(YD115)
"十二五"国家科技支撑计划项目(2011BAI04B06)
关键词
芡实
高效液相指纹图谱
柚皮素
主成分分析
聚类分析
Euryales Semen
HPLC fingerprint
naringerin
principal component analysis
cluster analysis
