摘要
目的:观察RANTES、FOXP3、ED-1在肾组织中的蛋白表达,探讨LEF对糖尿病肾病肾组织炎症反应及病理改变的影响。方法:35只雄性Wistar大鼠随机分为正常对照组N(n=10)、模型DN(n=13)、来氟米特组DD(n=12)。采用弗氏完全佐剂(CFA)和链脲佐菌素(STZ)制备糖尿病肾病模型。检测24 h尿蛋白定量,HE染色观察肾脏组织学改变,免疫组织化学方法检测肾组织RANTES、FOXP3、ED-1表达。结果:与对照组比较,模型组24 h尿蛋白排泄量显著上升(P<0.05),模型组大鼠肾组织肾小球肥大、肾小管上皮细胞变性或脱落、单核/巨噬细胞浸润、肾间质病变等病理变化明显;模型组大鼠肾组织内RANTES、FOXP3、ED-1表达均明显增强(P<0.05);与模型组比较,LEF治疗组大鼠24 h尿蛋白下降,肾组织RAN-TES、FOXP3、ED-1表达显著减少,肾脏病理改变有所减轻。结论:LEF可能是通过抑制RANETS、FOXP3、ED-1在肾组织中的表达,减少炎症细胞在肾组织中的浸润,对延缓糖尿病肾病的发展具有一定的作用。
OBJECTIVE To observe the expression of RANTES, FOXP3, ED-1 protein expression in the kidney of diabetic nephropathy rats,and study the effects of leflunomide （LEF） intertention on inflammatory response and pathological changes. METHODS 35 male Wistar rats were randomly divided into normal control group N （n = 10）, model group DN （n = 13）, le flunomide group DD （n = 12）. Rat model of diabetic nephropathy was induced by Freund＇s complete adjuvant （CFA） and streptozotocin （STZ）. 24 h urinary protein was determined. Histological changes in renal tissue were observed by hematoxlin eosin （HE） staining. RANTES, FOXP3 and ED-1 protein expression in renal tissue were detected by immunohistochemistry. RESULTS Compared with control group,24 h urinary protein excretion increased significantly （P〈0. 05） in model group, rats kidney in model group showed glomerular hypertrophy, tubular epithelial cell degeneration or loss, monocyte / macrophage infiltration, renal interstitial disease and other pathological changes significantly, RANTES, FOXP3, ED1 expressions in renal tissues of model group were significantly increased （P〈0. 05）;Compared with model group, 24 h urine protein and RANTES, FOXP3,ED-1 expressions and pathological changes in LEFtreated group were significantly reduced. CONCLUSION LEF probably inhibits RANETS,FOXP3, ED-1 expression and reduced inflammatory cell infiltration in renal tissues. LEF plays a certain role in delaying the development of diabetic nephropathy.
出处
《中国医院药学杂志》
CAS
CSCD
北大核心
2012年第9期667-671,共5页
Chinese Journal of Hospital Pharmacy
基金
贵州省科技厅基金资助[编号:黔科合J字2008(2003号)]
