摘要
采用营养液栽培,研究Ca(NO3)2和NaCl胁迫对黄瓜嫁接用砧木南瓜幼苗生长和抗氧化酶活性的影响,并用隶属函数法综合评价其耐盐性.结果表明:低浓度盐30mmol.L-1Ca(NO3)2和等渗的45mmol.L-1NaCl处理促进砧木幼苗生长;高浓度盐60、120mmol.L-1Ca(NO3)2和等渗的90、180mmol.L-1NaCl胁迫下,各砧木幼苗的生长和抗氧化酶系统均受到不同程度的抑制,其中,‘青砧1号’的盐害指数最小,生物量及超氧化物歧化酶(SOD)、过氧化物酶(POD)和过氧化氢酶(CAT)活性的下降幅度以及相对电导率的上升幅度均小于其他砧木.高盐Ca(NO3)2胁迫下,各砧木SOD、POD和CAT酶活性均高于等渗的NaCl,而盐害指数和相对电导率低于NaCl,表明Ca(NO3)2对砧木南瓜幼苗生长的危害小于NaCl.4个砧木品种的耐盐性顺序为‘青砧1号’>‘佐木南瓜’>‘丰源铁甲’>‘超霸南瓜’.
Abstract： Taking 4 different Cucurbita maxima x C. moschata rootstocks for cucumber （ Cucumis sativus ） as test materials, a solution culture experiment was conducted to study their growth and an- tioxidative enzyme activities under the stresses of Ca（ NO3 ）2 and NaCl, with the salt stress tolerance of the rootstocks evaluated by subordinate function. At 30 mmol ~ L^1 of Ca （NO3）2 or 45 mmol .L^1 of NaC1, the growth of the rootstock seedlings was improved ; but at 60 and 120 mmol.L^1 of Ca（ NO3 ）2 or 90 and 180 mmol ~ L-I of NaC1, the growth and the antioxidative systems of the seedlings were inhibited, and the salt injury index of ＇ Qingzhen No. 1 ＇ was the smallest, with the decrement of biomass and SOD, POD and CAT activities and the increment of relative conductance being significantly lower than those of the others. Under the stress of high concentration Ca（ NO3 ） 2, the SOD, POD and CAT activities of test rootstocks were higher, and the salt injury index and rela- tive conductance were lower, as compared with those under high concentration NaC1, suggesting that the damage of Ca（ NO3 ） 2 stress to cucumber-grafted rootstock were smaller than that of NaC1 stress. Among the 4 rootstocks, by ＇ Zuomu Nangua＇ , ＇ Fengyuan Qingzhen No. 1 ＇ had the strongest salt stress tolerance, followed Tiejia＇ , and ＇ Chaoba Nangua＇.
出处
《应用生态学报》
CAS
CSCD
北大核心
2012年第5期1311-1318,共8页
Chinese Journal of Applied Ecology
基金
国家重点基础研究发展计划项目(2009CB119000)
国家自然科学基金项目(30900995
31071831)
农业部现代农业产业技术体系建设专项(CARS-25-C-03)
教育部高校博士点基金项目(20100097110001)
江苏省农业三新工程项目[SX(2011)289]
江苏省高校优势学科建设工程项目资助
关键词
盐胁迫
白籽南瓜
抗氧化酶
隶属函数
salt stress
Cucurbita maxima ~ C. moschata
antioxidative enzyme
subordinatefunction.
