摘要
目的探讨抗独特型抗体(Ab2)2H4和5D3模拟抗原诱导免疫应答的能力。方法用Ab2免疫Balb/c小鼠获得抗血清 ,以ELISA检测其Ab3,WesternBlot分析其识别抗原的分子质量。用迟发型超敏反应(DTH)和淋巴细胞增殖试验 ,检测Ab2诱发细胞免疫反应的能力。结果2H4,5D3免疫同系动物产生的含有抗抗独特型抗体(Ab3)的抗血清 ,可特异性地与靶细胞结合。FC2(Ab1)和经2H4免疫的Ab3可识别相对分子质量(Mr)相同约68000的抗原 ,且不同于HNL5(Ab1)和5D3免疫的Ab3所识别的抗原(Mr80000)。在DTH试验中 ,所致小鼠足垫肿大的程度 ,2H4和5D3实验组均显著高于对照组。而在淋巴细胞增殖试验中 ,Ab2免疫鼠脾T细胞在体外对靶细胞或Ab2的再次刺激呈现明显的细胞增殖反应 ,而无关肿瘤细胞或抗体的刺激则不能引起细胞增殖。结论抗独特型抗体2H4和5D3具有鼻咽癌相关抗原的内影像 ,能模拟不同分子质量的鼻咽癌相关抗原诱导体液和细胞免疫应答。
Aim To Study the efficacy of monoclonal anti idiotype antibodies (Ab2)2H4 and 5D3 to induce humoral and cellular immune responses. Methods 2H4 and 5D3 were used to immunize mice. Ab3 in anti sera were tested by ELISA and Western blot. Cell mediated immunity to tumors induced by Ab2 was investigated by a delayed type hypersensitivity(DTH)response and mouse T cell proliferation assay. Results The anti sera derived from the immunized mice containing anti anti idiotype antibodies(Ab3) bound specifically to NPC cell line (HNE2). Using Western blot and immunostaining, Ab1(FC2 or HNL5) and Ab3 sera derived from mice immunized with Ab2 (2H4 or 5D3)recognized the antigenic determinants with the relative molecular mass(Mr)of 68 000 and 80 000, respectively. Mice immunized with 2H4 or 5D3 coupled with KLH occurred positive and specific DTH reaction after NPC cell stimulation. The results of mice T cells proliferation assay indicated that proliferarive response of the splenocytes in experimental groups was significantly higher than that in control groups. Conclusion Bearing the internal image of NPC associated antigen, anti idiotype antibodies(Ab2) 2H4 and 5D3 imitated antigen that vary in the relarive molecular mass. They could induce humoral and cellular immune responses in syngenetic mice and might substitute for NPC associated antigen as a good NPC candidate vaccine.
出处
《细胞与分子免疫学杂志》
CAS
CSCD
2000年第2期134-137,共4页
Chinese Journal of Cellular and Molecular Immunology
基金
国家自然科学基金!资助
No.39270706
关键词
抗独特型抗体
鼻咽癌
体液免疫
细胞免疫
ani idiotypic antibody
anti anti idiotypic antibody
delayed type hypersensitivity
cells proliferation
