摘要
目的 探讨载药骨基质明胶 (BMG)修复节段性骨缺损及防治术后感染的可能性。方法 将BMG溶于头孢唑林钠溶液 ,通过真空吸附、冻干等处理 ,制得载药 BMG。测定其体内、体外释药浓度及持续时间以及载药 BMG修复节段性骨缺损的能力。结果 载药 BMG体外对金黄色葡萄球菌的抑制作用为 2 2天 ,体内为 14天。载药 BMG体内释药时 ,局部组织浓度高 ,血浆浓度低 ;局部组织早期浓度高 ,以后为稳定的低浓度释放。载药BMG具有良好的修复骨缺损的能力。结论 载药 BMG既能持续释放有效浓度的抗生素 ,又具有良好的修复骨缺损的能力 。
Objective To explore the possibility of repair long segmental bone defects and preventing infection with cefazolin loaded bone matrix gelatin (C BMG). Methods\ C BMG was made from putting cefazolin into BMG by vacuum adsorption and freeze drying techniques. The sustaining period of effective drug concentration in vitro and in vivo was detected by inhabition bacteria, and the drug concentration in local tissues (bone and muscle) and plasma after implantation of C BMG was examined by high performance liquid chromatography(HPLC). Results\ The effective inhibition time to staphylococcus aureus of C BMG was 22 days in vitro, while 14 days in vivo. The drug concentration in local tissues(bone and muscle) were higher than that of plasma, and the drug concentration in local tissues was higher in early stage, later it kept stable low drug release. It suggested that C BMG had excellent ability to repair segmental long bone defects. Conclusion\ C BMG can gradually release cefazolin with effective drug concentration and has excellent ability to repair segmental long bone defects. It may be a novel method to repair segmental long bone defects and prevent infection after the operation.
出处
《中国修复重建外科杂志》
CAS
CSCD
2000年第3期162-165,共4页
Chinese Journal of Reparative and Reconstructive Surgery
基金
卫生部科学研究基金资助项目!(96-1-145)
关键词
载药骨基质明胶
骨缺损
感染
预防
Cefazolin loaded bone matrix gelatin Bone defect Infection
