摘要
目的 研究ATP敏感钾通道 (KATP)亚单位在大鼠组织中的表达。方法 逆转录多聚酶链式反应 (RT PCR)检测通道mRNA的表达。结果 左心室有Kir 6 1,Kir 6 2和SUR 2A的表达 ,大脑皮层中Kir 6 1,Kir 6 2 ,SUR 1,SUR2B均有表达 ,主动脉平滑肌有Kir 6 1,Kir 6 2 ,SUR 2B的表达 ,膀胱平滑肌中有Kir 6 1,Kir 6 2 ,SUR 2B的表达。结论 各组织的KATP组成有所不同 ,Kir 6 1、Kir 6 2和SUR 2B亚单位在多组织中广泛表达。
AIM To observe the expression of ATP sensitive potassium channels subunit in rat tissues. METHOD Reverse transcription polymerase chain reaction (RT PCR) was performed to detect the channels mRNA expression. RESULTS In left ventricle the Kir 6 1, Kir 6 2 and SUR 2A were expressed; Kir 6 1, Kir 6 2 and SUR 1, SUR 2B were all exprssed in cerebral cortex, and in aorta and bladder smooth muscles the Kir 6 1, Kir 6 2 and SUR 2B were expressed. CONCLUSION There are different subunit constitution of K ATP in different tissues, the Kir 6 1, Kir 6 2 and SUR 2B subunit are widely expressed in rat tissues.
出处
《中国药理学通报》
CAS
CSCD
北大核心
2000年第2期148-150,共3页
Chinese Pharmacological Bulletin
基金
国家自然科学基金资助课题 !No 3 9770 85 9
