摘要
目的:探讨下丘脑室旁核(hypothalamic paraventricular nucleus,PVN)注射GLP-1(胰高血糖素样肽-1)对糖尿病大鼠胃排空的影响及机制。方法:30只Wistar大鼠随机分为正常对照组(NC组)、糖尿病组(DM组)和GLP-1干预组(GLP-1组),每组各10只。DM组和GLP-1组腹腔注射链脲佐菌素,三组大鼠均PVN区埋置套管,恢复7d,GLP-1组微量注射0.5μg/0.5μl的GLP-1,NC组和DM组大鼠PVN区微量注射等体积生理盐水。甲基纤维素-酚红灌胃法检测胃排空;半定量RT-PCR检测大鼠下丘脑GLP-1RmRNA的表达。结果:DM组胃排空率较NC组明显升高(P<0.05),GLP-1组胃排空明显低于DM组(P<0.05),GLP-1组和NC组差异无统计学意义(P>0.05)。GLP-1组下丘脑GLP-1RmRNA的表达明显高于DM组和NC组(P<0.05),并与胃排空率成负相关(P<0.05)。DM组和NC组差异无统计学意义(P>0.05)。结论:PVN区注射GLP-1可以抑制糖尿病大鼠早期胃排空加速,作用机制可能和促进下丘脑GLP-1受体表达有关。
Objective： To investigate the effection of GLP-1 injection GLP-1 in paraventricular nucleus on the expression of GLP-1 receptor and gastric emptying in diabetic rats.Methods： Thirty Wistar rats were randomly divided into three groups： Group 1： normal control group（NC group）,Group 2： diabetic mellitus group（DM group） and Group 3： GLP-1 treated group（GLP-1 group）.All of rats were posited a cannula in PVN,the rats of Group 2 and Group 3 were induced by intraperitoneal injection of streptozotocin（STZ）.The rats of Group 3 were injected 0.5μg/0.5μl GLP-1 and isovolumic normal saline for Group 1 and Group 2 in PVN after 7-day recovery period following surgery.Gastric emptying was measured by intragastric administration of methyl cellulose-phenol red solution.The GLP-1 levels in the hypothalamus were measured by EISIA method,the expression levels of GLP-1R mRNA in the hypothalamus were measured by semi-quantitative RT-PCR.Results： The gastric emptying of Group 2 showed significantly accelerated compared with that Group 1（P〈0.05）.which decreased significantly in Group 3 compared to Group 2（P 0.05）,There was no statistical difference between Group 2 and Group 1（P〈0.05）.The expression of GLP-1 and GLP-1R mRNA in hypothalamus of Group 3 was increased significantly compared with in the other two groups and no difference between Group 2 and Group 1（P〈0.05）,which showed a negative correlation with decrease of gastric emptying（P〈0.05）.Conclusion： GLP-1 Injection in PVN delays acceleration of gastric emptying during the early stage of diabetic rats.The expression of GLP-1 receptor in the hypothalamus is promoted by GLP-1 may be the mechanism.
出处
《现代生物医学进展》
CAS
2012年第8期1452-1456,共5页
Progress in Modern Biomedicine
基金
Natural Science Foundation of Shang Dong province(ZR2009CM105)~~
关键词
糖尿病
室旁核
胃排空
胰高血糖素样肽-1
受体
Diabetes mellitus
Paraventricular nucleus
Gastric emptying
GLP-1
Receptor
