摘要
已知组织因子异常表达可启动血液凝固机制。用细胞发色底物法、ＲＴ－ＰＣＲ等方法分别从蛋白活性、基因表 达水平研究与动脉粥样硬化及高血压发病相关的肿瘤坏死因子、血管紧张素Ⅱ对血管内皮细胞组织因子表达的影响。 结果表明：肿瘤坏死因子α和血管紧张素Ⅱ均诱导血管内皮细胞组织因子的表达，并使其ｍＲＮＡ表达增加。此项研 究为进一步探讨动脉粥样硬化和高血压的共同发病机制打下基础。
Cell chromogenic substrate assay and RT-PCR method were applied to study the effects of tumor necrosis factor a and angiotensin Ⅱ on the expression of protein activity and mRNA of tissue factor. ECV304 endothelial cells were stimulated with tumor necrosis factor a and angiotensin Ⅱ. Results showed that tumor necrosis factor a and angiotenisn Ⅱ induced tissue factor activity. Tissue factor activity on ECV304 cell surface increased significntly after stimulated with 100U/mL tumor necrosis factor a for 6h (P<0.05 ) or with 10^-6 mol/L angiotensin Ⅱ for 12h (P<0.05 ). Moreover,the expression of TF mRNA increased compared with the control group. Results is of benefit to the further research on the common mechanism of atherosclerosis and hypertension.
出处
《基础医学与临床》
CSCD
2000年第2期30-33,共4页
Basic and Clinical Medicine
基金
国家自然科学基金!(批准号39730220)
关键词
TNF-Α
血管紧张素Ⅱ
血管内皮
组织因子
Tumor necrosis factor a
Angiotensin Ⅱ
Endothelial cell
Tissue factor
