摘要
目的:筛选补肾活血复方含药血清促SD大鼠骨髓间充质干细胞(MSCs)增殖和骨向分化最佳取血时间和浓度。方法:10月龄SD雌性大鼠30只,随机分为补肾活血复方低(11.6 g/kg)、中(34.8 g/kg)、高(104.4 g/kg)剂量组,每组10只。灌胃1次/d,连续12 d。分别于末次灌胃后的0.5、1.0、1.5、2.0、3.0 h取血,分离血清,然后将上述复方含药血清分别用不同添加浓度干预3月龄SD大鼠MSCs,以MTT法检测细胞增殖情况,以碱性磷酸酶(ALP)确定各组MSCs骨向分化情况。结果:补肾活血复方含药血清促MSCs增殖以复方低剂量灌胃12 d,末次灌胃1 h后取血,25%的添加浓度为佳;促MSCs骨向分化以复方高剂量灌胃12 d,末次灌胃1 h后取血,25%～30%的添加浓度为佳。结论:补肾活血复方含药血清能促进SD大鼠MSCs增殖及骨向分化。
Objective：To screen the optimal cell proliferation and osteogenic differentiation concentration of SD rats′ Mesenchymal stem cells（MSCs）affected by compound Bushenhuoxue（CB）contained serum.Methods：30 SD female rats,aged 10 months,were randomly divided into low-dose（11.6 g/kg）,medium dose（34.8 g/kg）and high dose CB group（104.4 g/kg）,10 rats in each group（qd,12 d）.Rats blood of three groups were taken respectively in 0.5,1.0,1.5,2.0,3.0 h after the last intragastric infusion,blood serum was separated,and different concentrations of CB contained serum were given to MSCs form SD female rats aged 3 months.MTT method was used to determine the effect of CB on MSCs proliferation.Alkaline phosphatase（ALP）was detected to determine the osteogenic differentiation of MSCs from each group.Results：The 25% concentration of CB contained serum collected from SD rats one hour after intragastric infusion with low dose CB was proved to be the most effective concentration on the proliferation of MSCs;The 25%-30% concentration of CB contained serum collected from SD rats one hour after intragastric infusion with high dose CB was proved to be the most effective concentration on the differentiations of MSCs into osteoblast.Conclusion：CB contained serum could promote the proliferation and osteogenic differentiation of MSCs.
出处
《中药材》
CAS
CSCD
北大核心
2012年第2期259-263,共5页
Journal of Chinese Medicinal Materials
基金
国家自然科学基金项目(30772885)
广东省自然科学基金重点项目(04105837)
广东省中医药管理局项目(2009176)
关键词
补肾活血复方
骨髓间充质干细胞
增殖
骨向分化
时效
量效
Compound Bushenhuoxue
Mesenchymal stem cells
Cell proliferation
Osteogenic differentiation
Time-effect relationship
Dose-effect relationships
