摘要
以9个肉兔纯系及其37个杂交组合为实验材料,采用测序和单链构象多态(SSCP)的分析方法探讨了肌肉生长抑制素(Myostatin,MSTN)基因的全部3个外显子区及部分5'调控区的遗传多态性,结果发现该基因5'调控区476位点存在1处T→C的单碱基突变,外显子区域没有发现突变位点。关联分析表明,该突变有增加活体重,提高胴体重、前腿重、背腰重、后腿重和皮重,同时降低肌肉滴水损失和熟肉率等有利遗传效应(P<0.05),结果提示位于MSTN基因上游调控区的该处突变有利于家兔的体躯发育,可资开发为用于家兔肉用性能选育的分子标记。
The polymorphism of myostatin（MSTN） gene in nine purelines and 37 crossbreds of rabbit was analyzed by sequencing and PCR-SSCP.There exons and partial 5′UTR were detected.Only one mutation（T→C） at base position 476 of 5′UTR was found among individuals in each line.The genetic effects of this polymorphism on growth traits,partial carcass traits and meat quality traits were investigated.The mutant allele had significant positive effects on live weight,carcass weight,forelegs weight,loin weight,ham weight and skin weight（P 0.05）,and reduced muscle drip loss and cooked rate.The results suggested that the variation in the upstream regulatory region of MSTN gene was beneficial to the body-development,and could be a potential molecular marker of meat quality traits selection in rabbit.
出处
《山东农业大学学报（自然科学版）》
CSCD
北大核心
2012年第2期247-251,共5页
Journal of Shandong Agricultural University：Natural Science Edition
基金
青岛康大兔业发展有限公司与山东农业大学合作课题"康大肉兔配套系育种"项目
