摘要
从工业废水污染的水体和污泥中,分离并筛选到6株对氯苯和苯酚类有机污染物高浓度耐受力的细菌菌株。6株降解细菌能在氯代苯和氯代酚作为唯一碳源的培养介质中生长,具有不同程度降解这类有机污染物的代谢能力。除2号和5号菌外,所筛选的其它4株降解细菌均含有一个或多个质粒。采用2,4D单加氧酶基因tfdA的保守引物和PCR扩增技术,证明在2号和6号菌的染色体和3号菌的大质粒上含有tfdA基因的同源序列。
Six chlorinated aromatic pollutant tolerant strains, which can grow in the chlorobenzenes, and phenols as the sole carbon source were isolated from the polluted soil or water samples. A study on the aerobic or microaerobic biodegradation of eight priority chlorinated aromatic pollutants was conducted using six strains. Strain No.1, 3,4 or 6 contained one or more plasmids. It was confirmed that sequence homologous to tfdA gene is chlorosome borne in strain No.2 or No 5 and megroplasmid borne in strain No.3 using universal primes of tfdA and PCR amplification.
出处
《高技术通讯》
EI
CAS
CSCD
2000年第2期21-23,17,共4页
Chinese High Technology Letters
基金
86 3计划资助项目!( 86 3 SZ 0 3 0 1)
关键词
氯代芳香族
污染物
降解细菌
工业废水
生物降解
chlorinated aromatic pollutants, bacterial strains, degradative plasmid
