摘要
目的了解上海地区汉族人群中神经肽Y(NPY)基因信号肽部位T1128C(Leu7Pro)单核苷酸多态性(SNP)的分布频率。方法利用聚合酶链反应(PCR)-连接酶检测反应(LDR)技术检测了867例上海地区汉族人群的NPY基因多态性。参照待测多态性位点所在DNA序列设计并合成1对引物及3条探针,先通过PCR获得含有待检测突变位点的基因片段,再进行PCR产物的LDR,根据测序电泳结果所显示的含荧光标记的LDR产物的片段长度来判断基因型别。结果 867例研究对象均显示NPY基因型为T1128T(Leu7Leu),未出现T1128C(Leu7Pro)或C1128C(Pro7Pro)。结论 NPY基因多态性存在明显的种族和地理差异,上海地区汉族人中不存在NPY T1128C(Leu7Pro)基因多态性,NPY基因T1128C多态性与上海地区汉族人群冠心病的发生、发展无相关关系。
Objective To investigate the distribution frequency of T1128C（Leu7Pro） single-nucleotide polymorphism（SNP） in neuropeptide Y（NPY） gene among Han population in Shanghai.Methods Polymerase chain reaction（PCR）-ligase detection reaction（LDR） was performed to detect the T1128C polymorphism in NPY gene among 867 Han people in Shanghai.A pair of primers and 3 probes were designed according to target DNA sequence.The LDR was performed after PCR amplification,and the genotypes were analyzed according to the length of fragments of LDR products on a sequencer by electrophoresis.Results All 867 subjects showed T1128T（Leu7Leu） but no T1128C（Leu7Pro） or C1128C（Pro7Pro） in the NPY gene.Conclusions There is difference of T1128C polymorphism in NPY gene among populations with different genetic and geographic backgrounds.There is no T1125C（Leu7Pro） polymorphism in NPY gene among Shanghai Han population.There is no correlation of T1128C polymorphism in NPY gene with coronary heart disease among Shanghai Han population.
出处
《检验医学》
CAS
2012年第4期304-307,共4页
Laboratory Medicine
关键词
神经肽Y基因
单核苷酸多态性
聚合酶链反应
连接酶检测反应
Neuropeptide Y gene
Single-nucleotide polymorphism
Polymerase chain reaction
Ligase detection reaction
