摘要
目的：测定Alzhuner病（AD）部分实验室指标，探讨其与临床的关系。方法：采用火箭电泳法（RIE），测定IgG和白蛋白含量，计算IgG鞘内合成率；运用酶联免疫吸附法（ELISA），测定脑脊液Tau和β－淀粉样蛋白（β－AP）的含量。结果：血清、脑脊液中白蛋白水平正常组最高，血清、脑脊液IgG水平AD组最高。但三级间均存在较大重叠。老年人脑脊液IgG鞘内合成率增高比例较大，无显著性差异。AD、MID患者脑存液Tau蛋白，β－AP含量均明显高于对照组，差别具有统计意义（P＜0．001），但二者差别无统计意义（P＞0．05）；结论：血清、脑存液中白蛋白水平和IgG水平在三组间无显著性差异；Tau蛋白和β－AP含量在AD患者脑誉液明显升高，在MID组亦增高，故单纯测定二者难以鉴别AD和MID，但可作为AD的实验室表现。在IgG鞘内合成率异常者中，Tau蛋白含量增高而β－AP含量降低者以AD最多、至于这样综合分析是否可为AD诊断提供实验室依据沿有待加大样本量进一步探讨。
Objective To explore a pecific experimental measurement of Alzheimer 's disease (AD). Methods: The levels of IgG and albumin of serum and cerebrospinal fluld was determined in 20 AD patients, 13 MID patients and 30 normal controls with RIE; calculate the 24 hours IgG synthe sis. The levels of tau and β- Amyloid protein in CSF was determined with ABC - ELISA. Results: The levels of albumin and IgG were overlap in AD,MID patients and normal cormal controls . There was no significent difference the three groups (P>0.05). There was no correlation between the levels of IgG synthesis and AD, The CSF- Tau and β- Amyloidprotein levels were aiedcently elevated in AD and MID patients, There are no significent difference between the two groups (P > 0.05). Conchusions: So simple detection is not a specific experimental diagnosis of AD, but it may be helpful to show the Blood - brain - Bartier damage in aged and to the diagnosis of AD when asssociated with other clinical investigations.
出处
《河南实用神经疾病杂志》
2000年第1期10-12,共3页
Henan Journal of Practical Nervous Diseases
关键词
IGG合成率
老年性痴呆
TAU蛋白
Β-AP
痴呆
Alzheimer's disease IgG synthesis Blood-brain-barrier Tau protein β-Amyloid protein ELISA
