摘要
目的 检测金黄色葡萄球菌 (金葡菌 )的耐药表型 ,指导临床合理用药。方法 对临床分离的金葡菌用琼脂筛选法区分耐甲氧西林金葡菌 (MRSA)和甲氧西林敏感金葡菌 (MSSA) ;用碘试管法和酸试管法测定 β -内酰胺酶 ,用K -B法进行药敏试验。结果 6 6株金葡菌中 ,MRSA占 45 .5 % ,产两种 β -内酰胺酶的百分率分别是84.8% (青霉素酶 )和 5 7.6 % (头孢菌素酶 ) ,MRSA和MSSA产酶率无明显差异。除万古霉素外 ,MRSA对 11种抗生素的耐药率介于 33.3%～ 10 0 % ,而多数MSSA对青霉素和红霉素耐药 ,对其它 9种抗生素的耐药率明显低于MRSA。结论 实验室应重视鉴别MRSA和MSSA ,加强金葡菌的耐药性监测。
Objectives To study the present resistant phenotypes of Staphylococcus aureus (S.aureus) and to instruct clinical rational application of antibiotics.Methods All isolated S.aureus were distinguished into methicillin resistant Staphylococcus aureus (MRSA) and methicillin sensitive staphylococcus aureus (MSSA) with salt agar screening method.β-lactamase determination were performed by Iodometric method and Acidimetric method.Sensitivity to 12 kinds of antibiotics were tested with K-B diffusion method.Results In the 66 isolates of S.aureus,MRSA accounted for 45.5% and the percentage of two β-lactamase positive strains were 84.8%(penicillinase) and 57.6%(Cephalosporinase) respectively.Determination of β-lactamase production showed no significant difference between MRSA and MSSA.Except vancomycin,MRSA showed multiresistance to 11 antibiotics with rates ranging from 33.3% to 100%.Most of MSSA were resistant to penicillin and Erythromycin,but the resistant rate to the other 9 antibiotics was lower than MRSA.Conclusions It is necessary to distinguish MRSA and MSSA strictly and to monitor their resistance to antibiotics seriously in every bacterial laboratory.
出处
《宁夏医学杂志》
CAS
2000年第2期75-76,共2页
Ningxia Medical Journal
关键词
金黄色葡萄球菌
药物耐药性
药敏试验
Staphylococcus aureus
Drug tolerance
Antibiotics sensitivity test
