摘要
目的探讨可溶性白细胞介素一２受体（ｓＩＬ－２Ｒ）和白细胞介素一８（ＩＬ－８）在小儿不同病因中枢神经系统（ＣＮＳ）感染急性期脑脊液（ＣＳＦ）中的变化规律。方法采用双抗体夹心ＥＬＩＳＡ法检测了２１例结核性脑膜炎结脑、１５例化脓性脑膜炎（化脑）和２０例病毒性脑膜炎患儿急性期ＣＳＦ中ｓＩＬ－２Ｒ和ＩＬ－８。结果结脑、化脑及病毒性脑膜炎急性期ＣＳＦ中ｓＩＬ－２Ｒ水平分别为（３５０±８１．２）Ｕ／ｍｌ，（１７４．５±５０．１）Ｕ／ｍｌ和（８０．１±２１．７７）Ｕ／ｍｌ，均明显高于正常对照组（２７．３７±１２．１９）Ｕ／ｍｌ（Ｐ＜０．０１）；ＩＬ－８水平分别为（２１８．８１±３２．５２）ｐｇ／ｍｌ、（３６０．８±６９．３８）ｐｇ／ｍｌ及（１０６．７５±３７．１６）ｐｇ／ｍｌ，明显高于正常对照组（４２．３７±１４．９２）ｐｇ／ｍｌ（Ｐ＜０．０１）；结脑、化脑及病毒性脑膜炎急性期ＣＳＦ中。结论ｓＩＬ－２Ｒ在结脑的发病机制中起重要作用，其在ＣＳＦ显著增高有助于结脑与病毒性脑膜炎、化脑的鉴别诊断；ＩＬ－８在化脑的发生发展中起重要作用，ＣＳＦ中ＩＬ－８检测有助于化脑与病毒性脑膜炎、结脑的鉴别诊断。
Objective To find out the change law of the soluble interleukin-2 receptor (sIL--2R) and interleukin--8 in the cerebrospinal fluid (CSF) from the children with acute central nervous system infections (CNSI) due to different etiology. Methods The concentration of sIL--2R and IL--8 were investigated in the CSF of 20 patients with acute tuberculosis meningitis (TBM), 15 patients with acute purulent meningitis (PM), 21 patients with acute viral meingitis (VM), utilizing a quantitative immunometric Sandwich enzyme immunoassay (ELISA). Results The CSF levels of sIL--2R and IL-8 were significantly higher in the patient group than in the control group (P<0.00l).The sIL--2R concentration was markedly higher in the CSF of patients with VM (p<0.01) and patients with PM (p<0.0l). The IL--8 level was extremely higher in the CSF of patients with PM (360.81+69.38pg/ml) than in that of patients with VM (P<0.00l) and patients with TMB (p<0.0l). Conclusion The CSF levels of sIL--2R may be helpful to distinguish TBM from PM and VM. The CSF level of IL--8 may he valuable in differenciating between VM and PM, TBM.
出处
《中国实验诊断学》
2000年第1期15-17,共3页
Chinese Journal of Laboratory Diagnosis
