摘要
目的:了解抗鼠科肉瘤病毒癌基因同源物B1(v-raf murine sarcoma viral oncogene homologue B1,BRAF)抗体在类风湿关节炎(rheumatoid arthritis,RA)诊断中的临床意义。方法:纳入RA患者112例、健康对照73例、其他风湿病患者112例,以重组人BRAF蛋白为抗原,采用酶联免疫吸附法(enzyme-linked immunosorbent assays,ELISA)检测患者血清中的抗BRAF抗体水平,并收集RA患者的临床资料,采用SPSS 13.0软件行统计学分析。结果:(1)抗BRAF抗体的阳性率在RA患者组为53.6%,明显高于正常对照组(4.1%,P<0.01)和除骨关节炎(34.2%)组以外的其他风湿病组(强直性脊柱炎11.8%,系统性红斑狼疮20.1%,原发性干燥综合征17.9%,P均<0.01);RA患者血清中抗BRAF抗体滴度也明显高于正常对照组和其他风湿病组(P均<0.01)。(2)抗BRAF抗体对RA诊断的敏感度和特异度分别为53.6%和84.3%。(3)类风湿因子、抗环瓜氨酸抗体、抗角蛋白抗体和抗核周因子抗体阴性RA患者的抗BRAF抗体阳性率分别为52.6%、38.2%、30.3%和31.0%。(4)RA患者的抗BRAF抗体水平与患者的年龄、病程、C反应蛋白水平呈负相关。结论:抗BRAF抗体有助于RA的诊断,并可作为其他自身抗体的补充。
Objective： To clarify the clinical significance of the antibody against v-raf murine sarcoma viral oncogene homologue B1（BRAF） in the diagnostic practice of rheumatoid arthritis（RA）.Methods： In the study,112 patients with RA,112 patients with other rheumatic diseases,and 73 healthy individuals were recruited.With recombinant human BRAF protein as antigen,we examined the level of anti-BRAF antibody in all the patients by enzyme-linked immunosorbent assay（ELISA）,The clinical data of the RA patients were collected simultaneously,and analysed statistically by using SPSS 13.0.Results： The positive rate of anti-BRAF antibody was 53.6% in the RA patients,which was significantly higher than that of the normal control group（4.1%,P0.01）and other rheumatic diseases groups（P all0.01）except osteoarthritis group.The titer of anti-BRAF antibody was also notably higher in the patients with RA than in other rheumatic diseases and normal control groups（P all 0.01）.The diagnostic sensitivity and specificity of anti-BRAF antibody for RA were 53.6% and 84.3% respectively.The positive rate of anti-BRAF antibody in rheumatoid factor,anti-cyclic citrullinated peptide antibody,antikeratin antibody,antiperinuclear factor negative groups were 52.6%,38.2%,30.3% and 31.0%respectively.It showed significant negative correlation between the titer of anti-BRAF antibody and patient＇s age,disease duration and the level of CRP.Conclusion： The anti-BRAF antibody contributes to the diagnosis of RA,and may act as a supplement of other autoantibodies.
出处
《北京大学学报（医学版）》
CAS
CSCD
北大核心
2012年第2期195-198,共4页
Journal of Peking University:Health Sciences
基金
国家自然科学基金(81030057
81072474)
北京市自然科学基金(7112143)资助~~
