摘要
目的探讨氧化应激对高糖诱导的牛视网膜毛细血管周细胞凋亡的影响。方法体外培养三代融合的牛视网膜血管周细胞，分别在对照组（5．5mmol／L）与高糖各组（15、25、35mmol／L）中孵育6d，透射电镜观察周细胞超微结构改变，TUNEL法检测培养后周细胞的凋亡情况，检测培养液丙二醛（MDA）浓度及超氧化物歧化酶（SOD）浓度。结果15mmol／L、25mmol／L与35mmol／L葡萄糖组凋亡率分别为（28．35±0．84）％、（40．43±0．93）％与（50．16±0．60）％，均明显高于对照组的（4．41±0．66）％，差异有统计学意义（F=21．97，P〈0．01）。高糖各组MDA浓度／SOD活力比值增加（F=13．73，P〈0．01）。周细胞凋亡率与MDA浓度／SOD活力比值呈正相关（r=0．893，P〈0．01）。结论氧化应激参与了高糖诱导的牛视网膜毛细血管周细胞凋亡的过程。
Objective To study the effects of oxidative stress on apoptosis of bovine retinal capillary pericytes（BRPs） under high glucose condition. Methods BRPs were cultured for 6 days respectively in control group（ 5.5 mmol/L） and in high glucose conditions （ 15,25,35 mmol/L）. The sub-cellular structure was observed under electron microscope. Contents of malondialdehyde （ MDA ） and superoxide dismutase （ SOD ） activities were detected respectively. Results The BRPs showed typical apoptotic morphograpy in the high glucose conditions with the apoptosis rates of（ 28. 35 ± 0. 84 ） %, （ 40.43 ± 0. 93 ） % and （ 50. 16 ± 0. 60 ） % in 15 mmol/L, 25 mmol/L and 35 mmol/L glucose condition, much higher than that of control group （4. 41 ± 0. 66）% （F = 21.97, P 〈 0. 01 ）. The MDA content/SOD activity of BRPs in high glucose groups were higher than those in the control group（ P 〈 0. 01 ）. The MDA content/ SOD activity was positively correlated with the apoptosis rate of BRPs （ r = 0. 893 ,P 〈 0. 01 ）. Conclusion Oxidative stress may participated in the apoptosis of bovine retinal capillary pericytes under high glucose condition.
出处
《中国综合临床》
2012年第5期466-468,共3页
Clinical Medicine of China
基金
国家中医药管理局中医药科学技术研究专项课题（06-07JP14）
