摘要
目的:考察了贵州都匀产铁包金不同部位总黄酮含量的差异。方法:以乙醇作为回流提取溶剂提取铁包金中总黄酮。以芦丁为对照品,采用紫外分光光度法测定铁包金中的总黄酮。结果:芦丁标准品溶液浓度在5～30μg·L-1与吸光度有良好线性关系,回归方程为Y=0.011 2X+0.041 9(r=0.999 1),平均加样回收率98.56%,精密度试验RSD值为0.279%,重复性试验RSD为0.634%,稳定性试验RSD 1.49%。以此方法测定,铁包金叶和茎部中黄酮含量分别为12.3%,1.42%,叶中总黄酮含量是茎部的的8.7倍。结论:贵州都匀产铁包金叶和茎部中黄酮含量存在较大差异,叶部位具有较大的药用开发价值和应用前景。
Objective： The content distribution of total flavonoids in different parts of Berchemia lineate from city of DuYun in Guizhou was investigated.Method：Flavonids in different parts of Berchemia Lineate（L）DC.were extracted by ethanol as solvent reflux,The total flavonoids were determined by spectrophotometry,rutin as control sample.Result：The absorbency and concentration of rutin have good linear correlation in the range of 5-30 μg·L-1.The regression equation was Y=0.011 2X＋0.041 9（r=0.999 1）,the average recovery rate was 98.56%,Accuracy experiment RSD 0.279%,repeatability test RSD 0.634%,stability test RSD 1.49%,the contents of total flavonoids in leaf and stem was respectively 12.30% and 1.42%,that the means content of total flavonoids in leaf is 8.7 times higher than that in stem.Conclusion： the content of total flavonoids in the leaf and in the stem has a big difference,thereby the leaf of B.lineate from Duyun in Guizhou has value to be developed and used.
出处
《中国实验方剂学杂志》
CAS
北大核心
2012年第9期115-117,共3页
Chinese Journal of Experimental Traditional Medical Formulae
关键词
铁包金
紫外分光光度法
总黄酮
Berchemia Lineate
UV spectrophotometer
total flavone
