摘要
目的建立千柏鼻炎片中盐酸麻黄碱含量的测定方法。方法采用反相高效液相色谱法(RP-HPLC法)测定千柏鼻炎片中盐酸麻黄碱的含量,SHIMADZU-ODS(150mm×4.6mm,5μm)色谱柱;甲醇∶水∶三乙胺∶磷酸(10∶90∶0.1∶0.1)为流动相;流速为1.0mL.min-1;柱温为室温;检测波长214nm。结果盐酸麻黄碱在0.010～0.100mg.mL-1内呈现良好的线性关系(r=0.999 9),平均回收率为99.66%,RSD=1.1%。结论 RP-HPLC法简便、稳定、准确、重复性好,可用于千柏鼻炎片的质量控制。
Objective To establish a reverse-phase high-pressure liquid chromatography（RP-HPLC） method for the determination of ephedrine hydrochloride in Qianbai Biyan tablets.Methods The chromatographic separation was achieved at room temperature on a SHIMADZU-ODS（150 mm×4.6 mm,5 μm） column at a flow rate of 1.0 mL·min-1.Mobile phase consisted of methanol,water,triethylamine and phosphonic acid in a 10∶90∶0.1∶0.1 ratio.The detection wavelength was set at 214 nm.Results The ephedrine hydrochloride showed a good linearity in the range of 0.010~0.100 mg·mL-1（r=0.999 9）.The average recovery was 99.66% and the RSD was 1.1%.Conclusion The established RP-HPLC is simple,reliable,accurate and reproducible for the determination of ephedrine hydrochloride,and can be used for quality control of Qianbai Biyan tablets.
出处
《南昌大学学报（医学版）》
CAS
2012年第2期14-16,共3页
Journal of Nanchang University：Medical Sciences
关键词
千柏鼻炎片
反相高效液相色谱法
盐酸麻黄碱
Qianbai Biyan tablets
high-pressure liquid chromatography
ephedrine hydrochloride
