摘要
目的：考察茖葱（ Ａｌｌｉｕｍ ｖｉｃｔｏｒｉａｌｉｓ Ｌ．）中的５ 种化合物对小鼠培养心肌细胞的作用。方法：制备小鼠的培养心肌细胞，利用微机控制的图像分析监视系统，考察茖葱中分离出的５ 种挥发性成分对培养心肌细胞的心率及振幅的作用。结果：其中二甲基二硫、甲基烯丙基三硫、甲基烯丙基硫醚和二烯丙基二硫对培养心肌细胞的心率和振幅均有明显的增强作用，并对Ｃａ２ ＋ＡＴＰ酶有明显的抑制作用，而不含硫的２戊酮对心率、振幅、Ｎａ＋ ，Ｋ＋ＡＴＰ 酶及Ｃａ２＋ＡＴＰ酶均无影响。结论：含硫化合物引起的正性肌力作用可能与它的酶抑制作用有关。
AIM: To study the effects of volatile sulfur compounds isolated from caucas ( Allium victorialis L.) on spontaneous beating of cultured mouse myocardial cells. METHODS: Cultured mouse myocardial cells were prepared. The effects of five compounds from caucas on the rate and amplitude of beating of cultured myocardial cells were examined by using microcomputer driven image analyzing system. RESULTS: Of these compounds, dimethyl disulfide(DMDS), methyl allyl trisulfide(MATS), methyl allyl sulfide(MAS) and diallyl disulfide(DADS) appreciably increased the beating rate and beating amplitude, and significantly inhibited Ca 2+ ATPase activity, but non sulfur 2 pentanone did not affect them. CONCLUSION: The positive inotropic effect on cultured myocardial cell sheets induced by sulfur compounds may be correlated with its inhibition on enzyme.
出处
《药学学报》
CAS
CSCD
北大核心
2000年第1期4-6,共3页
Acta Pharmaceutica Sinica
关键词
心肌细胞
Ge葱
含硫化合物
细胞培养
药理作用
cultured myocardial cell
spontaneous beating
positive inotropic effect
Allium victorialis L.
sulfur compounds
