摘要
目的 研究牛磺酸是否能抑制STZ诱发的糖尿病性白内障方法 将30只Wistar大鼠平均分为3组,定期用裂隙灯照相显微镜和微量快速血糖仪检测各组动物,实验结束(12周)用TUNEL法检测各组晶状体上皮细胞凋亡。结果 STZ组产生了持续和长时间的高血糖,8～12周有80%晶状体发生白内障,几乎所有细胞呈现凋亡。相反,4%牛磺酸组则迅速阻断了高血糖形成,如同对照组一样,晶状体一直保持透明,仅有极少细胞凋亡。结论 4%牛磺酸能完全抑制糖性白内障发生,其主要作用可能与其抗氧化功能和抑制高血糖有关。
ObjectiveTo evaluate whether the taurine can attenuate cataract development in streptozotocin (STZ) induced diabetic rats.MethodsThe experiments were performed on male Wistar rats,body weight 180～200*!g,devided into the following groups:group one for control(10 rats),each rat receiving 50*!mmol/L citrate buffer,pH4 5;group two(10 rats),treated with STZ,following a 12 h fast,each rat received a single intraperitoneal(i.p.) injection STZ at a dose of 45*!mg/kg body weight in 50*!mmol/L citrate buffer,pH 4 5 and received a saline injection once daily for 12 weeks (i.p.5*!ml/kg body weight);group three (10 rats)was treated with STZ (same as group two)and received 4% taurine injection once daily for 12 weeks (i.p.5*!ml/kg body weight).Each rat was checked for cataracts once a week using a slit lamp microscope until 12 weeks after STZ administration,at 4,30,60 and 90*!d,tail blood was collected for glucose determination.Furthermore,the lens epithelial cell apoptosis from the rats of three groups were analyzed by the in situ TUNEL kit assay at the end of 12 weeks.Results(1)Control group didn′t show high blood sugar levels;STZ plus saline group produces a sustained and prolonged hyperglycemia in the rats;STZ plus 40% taurine group rapidly reduced a hyperglycemia to near normal value( P >0 05).(2)Control group and STZ plus taurine group:their lenses were completely transparent,throughout entire a period of experiment;but STZ plus saline group:by week 4,40% of rats exhibited vacuolar stage,by week 8 and 12,80% of the rats showed cataracts.(3)There were very few lens epithelial cells apoptosis with taurine treated,which were similar to the control rats.In contrast,almost all of the lens epithelial cell become apoptotic cell in STZ diabetic rats.ConclusionTaurine is an antioxidant,4% taurine can completely suppresses the cataract formation in STZ diabetic rats.Its main effect may be related to its antioxidative function and the inhibition of hyperglycemia.
出处
《眼科研究》
CSCD
2000年第1期38-40,共3页
Chinese Ophthalmic Research
基金
国家自然科学基金!资助 (基金编号 :39770 786)
