摘要
目的研究刺五加精制皂苷的体外抗人乳腺癌细胞MCF-7活性。方法香草醛-高氯酸比色法测定刺五加总皂苷含量,经大孔树脂D101梯度洗脱和丙酮沉淀纯化皂苷;MTT法研究该皂苷对乳腺癌细胞MCF-7增殖的作用。结果刺五加总皂苷含量为64.75 mg/g;纯化得到的乳黄色精制皂苷,其对乳腺癌细胞MCF-7的半抑制浓度IC50为29.57 g/ml,且呈剂量依赖性。结论刺五加皂苷具有很强的抑制人乳腺癌细胞MCF-7增殖作用。
ObjectiveTo investigate the antiproliferation effect of the purified saponins in Acanthopanax senticosus（Rupr.etMaxim.） Harms on human breast cancer cell line MCF-7 in vitro. MethodsThe saponins content was determined by Vanillin-perchloric acid colorimetry and furtherly purified by microporous resin D101 absorption technology and precipitated with acetone.MTT assay was used to investigate inhibitory growth effect of the purified saponins on MCF-7 cells. ResultsThe total content of tolal saponins was 64.75 mg/g compared with the standard syringin.The canary yellow refined saponins significantly inhibited the proliferation of human breast cancer MCF-7 cell lines in a dose-dependent manner and the IC50 was 29.57 g/ml. ConclusionThe saponins of Acanthopanax senticosus（Rupr.etMaxim.） Harms has a certain inhibition activity on the growth of breast cancer cells in vitro.
出处
《时珍国医国药》
CAS
CSCD
北大核心
2012年第4期926-927,共2页
Lishizhen Medicine and Materia Medica Research
基金
中国科学院知识创新项目(No.KZCX2-YW-443)
国家"十一五"科技支撑计划资助项目(No.2008BAD98B08)
关键词
刺五加
皂苷
香草醛-高氯酸比色法
抗乳腺癌细胞活性
Acanthopanax senticosus（Rupr.etMaxim.） Harms
Saponins
Vanillin-Perchloric acid Colorimetry
Anti-breast cancer cell activity
