摘要
目的 探索缺血损伤在同品系大鼠腹主动脉移植硬化中的作用。方法 将９０ 只大鼠随机分为３ 组： Ａ 组为同品系（ Ｗｉｓｔａｒ ｔｏ Ｗｉｓｔａｒ） 移植缺血小于３０ 分钟组； Ｂ 组为同品系移植缺血４ 小时组； Ｃ 组为异品系（ＳＤ ｔｏ Ｗｉｓｔａｒ） 移植缺血小于３０ 分钟组。于移植术后１５ 、３０ 及６０ 天切取植入的腹主动脉进行光镜和电镜检查，应用ＭＩＡＳ３００ 计算机图像分析系统自动测量移植腹主动脉管腔面积、内膜面积和中膜面积。结果 Ａ 组术后６０ 天动脉内膜轻度增厚，但Ｂ 组和Ｃ 组术后６０ 天均有明显管腔面积缩小和内膜增厚现象，与Ａ 组比较差异有显著性意义（ Ｐ＜ ０ ．０５） 。病理检查结果表明，Ｂ 组和Ｃ 组增生的内膜均由单核／ 巨噬细胞和平滑肌细胞构成，但前者无中膜层平滑肌坏死及弹力膜断裂现象。结论 缺血／ 再灌注损伤是移植动脉硬化的一个重要因素。
Objective To investigate the effect of cold ischemia on the development of transplant arteriosclerosis (TA) in rat aortic isografts. Methods Aorta grafts from SD and Wister rats were stored in a cold perfusion solution for 0.5 hours and 4 hours respectively before being orthotopically transplanted to Wister recipients. After observation times ranging from 15 to 60 days, the grafts were examined by using histological and electron microscopy techniques. Regional changes in the lumen, intima and media layers were measured by using an image analysis system. Results Partial intima thickings were showed in control isografts at 60 day post transplantation. Pronounced intima thickings were seen in experimental isografts and control allografts at the same time. The thicking neointimas consist mainly of monocyte/macrophage and smooth muscle cells (SMC). The broken interior elastic lamina (IEL) and necrosis SMC in media were detected in allogenic grafts. Conclusion The damage due to prolonged cold ischemia time is sufficient to cause pronouced graft arteriosclerosis.
出处
《中国普外基础与临床杂志》
CAS
2000年第1期19-21,共3页
Chinese Journal of Bases and Clinics In General Surgery
关键词
缺血
再灌注损伤
移植物
动脉硬化
大鼠
Ischemia/reperfusion injury Transplant arteriosclerosis Rat
