摘要
本文采用一种新的非同位素标记物Digoxigemin通过随机寡核苷酸引物法(RHP)制成Digoxigenin-HBV DNA探针,用于斑点杂交法检测血清标本中的HBV DNA,经敏感性试验证明该探针检测HBV DNA的灵敏度与^(32)P探针相近,而比生物素探针灵敏度高出近100倍,经与^(32)P-HBV DNA探针同时对126份临床标本进行检测比较,结果符合率达95.24％、对HBeAg阳性血清HBV DNA检出率高达86.49％,而抗HBeAg阳性血清HBV DNA检出率仅为10.20％。经过试验观察还证明,此探针检测HBV DNA的特异性高,重复性好,背景显色也较低。由于此探针既有^(32)P探针的高灵敏性,又具有生物素探针易保存、无放射性损伤等优点,因而具有较高的应用价值。
Anovel non-radioactive probe labelled with the hapten digoxigenin was prepared by random hexanu-cleotide primer (RHP) ,and this probe was used by dot-hybridization in serum specimens for the detection of HBV DNA. It was found that its sensitivity was equae to 32p-labelled probe,and about 100-fold more than biotinylated probe. 126 clinical specimens were detected by both 32p-and digoxigenin HBV DNA probe. The coincidence rate was 95. 24%. The positive rate of HBV DNA in HBeAg positive serum was 86. 49%, whereas the anti-HBe positive serum was 10. 20 %. Moreover, digoxigenin-HBV DNA probe was highly specific , stable and of low back-ground colour. Digoxigenin-HBV DNA probe has mamy advantages over 32p-and biotin labelled probes which make it ideal for both research and diagmostic laboratories.
出处
《中国人兽共患病杂志》
CSCD
北大核心
1990年第3期9-12,共4页
Chinese Journal of Zoonoses
关键词
乙肝病毒
DNA
探针
斑点杂交
Random hxeanucleotide primer,Digoxigenin-HBV DNA probe,Dot-hybridization, HBV DNA,
