期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

接头蛋白c-Crk单克隆抗体的制备和鉴定 被引量：2

Preparation and Identification of Monoclonal Antibody against Adaptor Proteins(c-Crk)
在线阅读 下载PDF
导出
摘要 目的制备接头蛋白c-Crk单克隆抗体。方法以c-Crk为抗原,免疫Balb/c小鼠,用杂交瘤技术建立稳定分泌抗c-CrkmcAb的杂交瘤细胞,用ELISA,western-blot等鉴定其生物活性。结果获得两株能稳定分泌c-CrkmcAb的杂交瘤细胞(3C7、3G11),亚类均为IgG1;腹水效价为1∶105;western-blot、免疫组织化学分析和ELISA检测证实两株mcAb均与c-Crk有较高的特异性。结论本实验成功制备了两株抗c-Crk特异性的mcAb,可用于c-Crk免疫检测方法的建立。 Objective To develop the methods for preparation of monoclonal antibody （McAb）against c-Crk. Methods Take c-Crk as antigen, immunize Balb/c mouse, establish hybridoma cells which stably secrete anti- c-Crk mcAb by hybridoma technique, use ELISA, western-blot etc. to indentify its living creature activity. Result Two stub c-Crk mcAb （3C7, 3G11） were acquired, and both subtype belong to IgG 1. The Ascites titer is 105. Re- sult from Western-blot, immunohistochemistry analysis and ELISA methods confirmed that the two stub mcAbs all have higher specificity with c-Crk. Conclusion This experiment successfully made two anti- c-Crk mcAb, which can be used for establishment of c-Crk immunity examination method.
作者 田方艳 黄欢 周文婷 曹辉 张培湘
机构地区 湖南远泰生物技术有限公司
出处 《肿瘤药学》 CAS 2011年第5期431-433,共3页 Anti-Tumor Pharmacy
关键词 接头蛋白c-Crk 单克隆抗体 WESTERN-BLOT Adaptor Proteins （c-Crk） Monoclonal antibody western-blot
分类号 R392.11 [医药卫生—免疫学] Q511 [生物学—生物化学]
  • 相关文献

参考文献12

  • 1朱进,胡孝渠,郭贵龙,尤捷,黄奇迪,张筱骅.乳腺癌组织中Crk蛋白的表达及其临床意义[J].实用肿瘤杂志,2009,24(5):446-449. 被引量：1
  • 2阴传敏,姚珍薇.信号接头蛋白c-Crk与恶性肿瘤关系的研究进展[J].重庆医学,2008,37(2):191-193. 被引量：3
  • 3翟波,廖侃.接头蛋白c-Crk的结构和功能[J].生命的化学,2003,23(3):198-201. 被引量：4
  • 4Kobashigawa Y,Sakai M,Naito M,et al.Structural basis for the transforming activity of human cancer-related sig-naling adaptor protein CRK. Nature Structural and Molecular Biology . 2007
  • 5Miller CT,Chen G,Gharib TG.Increased c-Crk proto-oncogene expression is associated with an aggressive phe-notype in lung adenocarcinomas. Oncegene . 2003
  • 6Mortazavi F,Dubinett S,Rettig M.c-Crk proto-oncogenec ontributes to transcriptional repression of p120-catenin in non-small cell lung cancer cells. Clinical and Experimental Metastasis . 2011
  • 7Sarkar P,Saleh T,Tzeng SR,et al.Structural basis for regu-lation of the Crk signaling protein by a proline switch. Nat Chem Biol . 2011
  • 8Sarkar P,Saleh T,Tzeng SR,et al.Structural basis for regu-lation of the Crk signaling protein by a proline switch. Nat Chem Biol . 2011
  • 9Hiroshi N,Shinya T,Tsuda M.Molecular and immunhisto-chemical analysis of signaling adaptor protein Crk in human cancers. Cancer Letters . 2002
  • 10Takino T,Nakada M,Miyamori H,et al.CrkI adapter protein modulates cell migration and invasion in glioblastoma. Cancer Research . 2003

二级参考文献50

  • 1Miller CT, Chen G, Gharib TG, et al. Increased CCRK proto-oncogene expression is associated with an aggressive phenotype in lung adenocarcinomas[J]. Oncogene, 2003,22 (39) : 7950- 7957.
  • 2Nishihara H,Tanaka S, Tsuda M, et al. Molecular and immunohistochemical analysis of signaling adaptor protein Crk in human cancers[J]. Cancer Lett,2002, 180(1):55-61.
  • 3Takino T, Nakada M, Miyamori H, et al. Crk I adapter protein modulates cell migration and invasion in glioblastoma [J]. Cancer Res, 2003, 63 (9): 2335- 2337.
  • 4Mayer BJ, Hamaguchi M, Hanafusa H.Characterization of p-17gag-crk, a novel oncogene product with sequence similarity to a putative modulatory domain of protein-tyrosine kinases and phospholipase C [J]. Cold Spring Harb Syrup Quant Biol, 1988, (53 Pt 2),907-914.
  • 5Menard S,Balsari A,Casalini P,et al. HER-2 positive breast carcinomas as a particular subset with peculiar clinical behaviors[J]. Clin Cancer Res, 2002,8(2) : 520 -525.
  • 6Buday L. Membrane-targeting of signalling molecules by SH2/SH3 domain-containing adaptor proteins [J]. Biochem Biophys Acta,199,9,14122(2):187 -204.
  • 7Iwahara T, Akagi T, Fujitsuka Y, et al. CrklI regulates focal adhesion kinase activation by making a complex with Crk-associated substrate,p130Cas[J]. Proc Natl Acad Sci USA,2004,101(51) : 17693- 17698.
  • 8Kiyokawa E, Hashimoto Y, Kobayashi S, et al.Activation of Racl by a Crk SH3-binding protein, DOCK180 [J].Genes Dev, 1998,12(21) : 3331- 3336.
  • 9Gotoh T, Hattori S, Nakamura S, et al. Identification of Rap1 as a target for the Crk SH3 domain-binding guanine nucleotide-releasing factor C3G[J]. Mol Cell Biol, 1995,15(12) :6746-6753.
  • 10Iwahara T,Akagi T,Shishido T,et al. CrkⅡ induces response factor activation and cellular transformation through its function in Rho activation [J]. Oncogene, 2003,22 (38) : 5946- 5957.

共引文献5

同被引文献32

  • 1郭坤,刘银坤,周海君,代智,陈洁,孙瑞霞,孙强玲,卢雯静,康晓楠.热休克蛋白27在转移潜能不同人肝癌细胞系中的表达及机制研究[J].生物化学与生物物理进展,2007,34(7):738-745. 被引量：9
  • 2Rupik W,Jasik K,Bembenek J. The expression pat-terns of heat shock genes and proteins and their role during vertebrate's development[J].{H}Comparative Biochemistry and Physiology A-MOLECULAR AND INTEGRATIVE PHYSIOLOGY,2011,(04):349-366.
  • 3Lanneau D,Wettstein G,Bonniaud P. Heat shock proteins:cell protection through protein triage[J].Scien-tific World Journal,2010.1543-1552.
  • 4O'Reilly AM,Currie RW,Clarke DB. HspB1(Hsp 27)expression and neuroprotection in the retina[J].{H}Molecular Neurobiology,2010,(02):124-132.
  • 5Kostenko S,Moens U. Heat shock protein 27 phosphory-lation:kinases,phosphatases,functions and pathology[J].{H}Cellular and Molecular Life Sciences,2009,(20):3289-3307.
  • 6Doshi BM,Hightower LE,Lee J. HSPB1,actin filament dynamics,and aging cells[J].{H}Annals of the New York Academy of Sciences,2010.76-84.
  • 7Calderwood SK,Ciocca DR. Heat shock proteins:stress proteins with Janus-like properties in cancer[J].Int J Hy-perthermia,2008,(01):31-39.
  • 8Uozaki H,Ishida T,Kakiuchi C. Expression of heat shock proteins in osteosarcoma and its relationship to prognosis[J].{H}Pathology Research and Practice,2000,(10):665-673.
  • 9Guo K,Kang NX,Li Y. Regulation of HSP27 on NF-κB pathway activation may be involved in metastatic hepatocellular carcinoma cells apoptosis[J].{H}BMC Cancer,2009.100.
  • 10Thanner F,Sütterlin MW,Kapp M. Heat shock protein 27 is associated with decreased survival in node-negative breast cancer patients[J].{H}Anticancer Research,2005,(3A):1649-1653.

引证文献2

二级引证文献2

肿瘤药学
2011年 第5期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部