摘要
目的探讨核转录因子κB(NF-κB)p65蛋白和血管内皮生长因子(VEGF)在肝癌组织中的表达及临床意义。方法用免疫组化SP法研究HCC30例、肝硬化组织10例和正常肝组织6例中NF-κBp65、VEGF的表达。结果 HCC组织中,VEGF的表达与包膜完整、肝内血管癌栓及淋巴结转移相关(P<0.05),而与年龄、性别、肿瘤大小、结果数目、组织病理学分级及肝硬化背景无关(P>0.05);NF-κBp65表达与肿瘤大小、组织病理学分级、肝内血管癌栓及淋巴结转移(P<0.05)相关,而与年龄、性别、结果数目、包膜完整、肝硬化背景无关(P>0.05);NF-κBp65与VEGF表达呈正相关(r=0.962,P<0.01)。VEGF在HCC癌旁组织中表达最高,而NF-κBp65以HCC组织表达最高。结论 NF-κBp65介导的VEGF异常表达可能在肝癌发生、进展和转移中独立发挥作用。
Objective To investigate the significance of the expression of NF-r,B p65 and VEGF proteins in heap to cellular carcinoma and their correlation. Method The expression of NF-kB p65 and VEGF w as de- tected in 30 cases of HCC, 10 cases of cirrhotic and 6 cases of normal liver tissues by using SP immunohistochemi- cal methods. Results In HCC, the VEGF, highly expressed in precancerous tissues, was correlated with tumor capsule, hepatic vein tumor thrombosis and lymphaden transfer （P〈0.05）, but not with age, sexuality, tumor size,the number of nodules, pathologic grade and cirrhosis background （P〉0.05）. The NF-r,B p65, highly expressed in carcinoma, was correlated with tumor size, pathologic grade, hepatic vein tumor thrombosis and lymphaden transfer （P〈0.05）, but not with age, sexuality, the number of nodules, tumor capsule and cirrhosis background （P〉0.05）.Pearson correlation analysis revealed that the NF-kB p65 expression was positively correlated with VEGF expres- sion in HCC （r=0.962）. Conclusion The aberrant expression of NF-kB p65 and VEGF may play a role in occur- rence, progression and intra hepatic metastasis of heap to cellular carcinoma.
出处
《肿瘤药学》
CAS
2011年第3期173-176,共4页
Anti-Tumor Pharmacy
关键词
肝细胞癌
核转录因子ΚB
内皮生长因子
免疫组织化学
Hepatic Cellular Cancer
, Nuclear Factor r.B
Endothelial Growth Factors
Immunohistochemistry
