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KiSS-1基因转染对骨肉瘤MG-63细胞增殖的抑制作用 被引量：1

KiSS-1 gene transfection inhibits the proliferation of osteosarcoma MG-63 cells
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摘要 目的探讨KiSS-1基因转染对骨肉瘤MG-63细胞株增殖的影响及其可能机制,为骨肉瘤的基因治疗提供实验室依据。方法将KiSS-1基因转染至骨肉瘤MG-63细胞株,应用免疫组化法检测MG-63-KiSS-1蛋白表达,CCK-8法检测细胞增殖情况,流式细胞术检测各期细胞比例。结果 MG-63-KiSS-1细胞KiSS-1蛋白高表达、增殖明显减慢、G2/M期细胞比例明显增高。结论 KiSS-1基因转染MG-63细胞后可抑制其增殖,其机制可能与阻滞细胞周期于G2/M期有关。 Objective To investigate the influence of KiSS-1 transfection on the proliferation of osteosarcoma cell,and to explore the feasibility of gene therapy for osteosarcoma.Methods A eukaryotic expression vector carrying the KiSS-1 gene（rAAV-KiSS-1）was transduced into MG-63 cells with Lipofectamine2000;Immunohistochemistry was applied to analyse KiSS-1 expression of MG-63-KiSS-1 cells;CCK-8 was used to examine cell proliferation and Flow cytometry was used to analyse the cell percentage of each cycle.Results MG-63-KiSS-1 cells highly expressed KiSS-1 protein.The cell proliferation was slow down obviously and the cell percentage of G2/M increased after rAAV-KiSS-1 infection.Conclusion The transfection of KiSS-1 gene inhibits the MG-63 cells proliferation,and the mechanism may be relate to blocking the cell cycle at G2/M period.
作者 林晋 黄宇福建医科大学附属第一医院 林建华
机构地区 福建卫生职业技术学院 福建医科大学附属第一医院
出处 《福建医药杂志》 CAS 2011年第6期83-85,共3页 Fujian Medical Journal
基金 福建医科大学苗圃科研基金(2010mp015)
关键词 KISS-1基因 骨肉瘤 MG-63细胞 增殖 转染 流式细胞术 KiSS-1 gene osteosarcoma MG-63 cell proliferation transfection flow cytometry
分类号 R738.1 [医药卫生—肿瘤]
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参考文献10

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二级参考文献19

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福建医药杂志
2011年 第6期

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