摘要
依据化学仿生原理,将整体药物研究与分子药物研究法(以微量元素为指标)相结合,设计体外仿生消化法用于蕨类植物分析前处理。以单层脂质体为细胞膜模型,萃取分离食糜中微量元素形态(单层脂质体亲合态、水溶态);根据单层脂质体亲合态含量对微量元素的生物可给性和危险性进行评价。比较单层脂质体结合亲和态金属在胃、肠中浓度,可得金属主要吸收部位。沙椤茎、叶和蜈蚣草茎、叶成年人摄入安全剂量分别为210.4、153.5、828.2、367.5 g/day,最大摄入剂量分别为864.1、1208.8、2067.1、1977.3 g/day。
A new pre-treatment technology,in vitro bionic digestion was designed,according to：（a） chemical bionic stomach and intestine,（b） whole medical effect,and（c） methods in molecular medicine,using trace metals as indicator.Monolayer liposome was used as biomembrane model.Monolayer liposome-water system was adopted to study the metal distribution in the chymes.Monolayer liposome-soluble metals（MLSMs） and water-soluble metals were used for speciation analysis of trace elements（Fe,Mn and Zn）.The MLSMs content was an important and accurate criterion for metal bioavailability and risk assessment.Based on the concentration of MLSMs,the main adsorption site of metal was proposed.The safe dosage and tolerable uptake level of the stem and leaves of A.spinulosa and P.vittata for adults were proposed as 210.4,153.5,828.2,367.5 g/day and 864.1,1208.8,2067.1,1977.3 g/day,respectively.
出处
《分析科学学报》
CAS
CSCD
北大核心
2012年第2期177-181,共5页
Journal of Analytical Science
基金
国家自然科学基金(No.20775067
21175115)
关键词
体外仿生消化
仿生萃取
蕨类植物
微量元素
生物可给性
In vitro bionic digestion
Bionic extraction
Pteridophyte
Trace element
Bioavailability assessment
