摘要
目的初步探讨有宫颈癌家族史的妇女人类白细胞抗原（HLA）-DRBl等位基因多态性的分布情况，为宫颈癌高危人群的检测及追踪提供理论依据。方法采用聚合酶链反应-序列特异性引物法检测1000例新疆和田地区墨玉县喀尔赛乡维吾尔族妇女血液中HLA-DRBl的13个等位基因。结果等位基因HIJA-DRBl。15在有宫颈癌家族史、母亲有宫颈癌病史和其他亲属有宫颈癌病史的妇女中出现的频率分别为17．3％、18．0％和17．O％，均明显高于无宫颈癌家族史的妇女（9．7％，均P〈0．05）。HLA-DRBl＊04在有宫颈癌家族史和除了母亲以外其他亲属有宫颈癌病史的妇女中出现的频率分别为16．8％和20．7％，明显高于无宫颈癌家族史的妇女（12．7％，均P〈0．05）。HIJA-DRBls03在有宫颈癌家族史和在除了母亲以外其他亲属有宫颈癌病史的妇女中出现的频率分别为2．6％和1．1％，明显低于无宫颈癌家族史的妇女（6．3％，均P〈0．01）。HLA-DRBl。12在有宫颈癌家族史和母亲有宫颈癌病史的妇女中出现的频率分别为2．3％和1．5％，明显低于无宫颈癌家族史妇女（5．7％，均P〈0．05）。HIA-DRBl}14在有宫颈癌家族史和母亲有宫颈癌病史的妇女中出现的频率分别为5．4％和3．O％，明显低于无宫颈癌家族史妇女（8．4％，均P〈0．05）。结论新疆墨玉县妇女有宫颈癌家族史者HLA-DRBl等位基因的分布与南疆维吾尔族宫颈癌妇女的HLA-DRBl等位基因的分布有相同点也存在一定差异。HLA易感基因及保护基因的研究在维吾尔族宫颈癌高发区宫颈癌高危人群及易感人群的检测中起一定的作用。
Objective To study the distribution of HLA-DRB1 allele polymorphism in Uyghur women with family history of cervical cancer, and provide theoretical evidence for detection and follow-up of high risk persons for cervical cancer by detection of HLA-DRB1 allele polymorphism. Methods The HLADRB1 13 alleles were detected in 1000 Uyghur women, all from Hotan Moyu county Karsay village by using polymerase chain reaction sequence-specific oligonucleotide （PCR-SSO） assay. Results The frequencies of HLA-DRB1 ＊ 15 in women with family history of cervical cancer （ 17.3% ） , mother （ 18.0% ） and other relatives except mother （ 17.0% ） who had suffered from cervical cancer were significantly hi^her than that in the control group （9.7%, all P 〈0.05）. The frequencies of HLA-DRB1 04 in women with family history （ 16.8% ） and other relatives except mother （20.7%） were significantly higher than that in the control group （ 12.7%, all P 〈0.05）. The frequencies of HLA-DRB1 ＊ 03 in women with family history （2.6%）and other relatives except mother （ 1.1% ） were significantly lower than that in the control group （6.3% , all P 〈0.01）. The frequencies of HLA-DRB1 ＊ 12 in women with family history of cervical cancer （2.3%） and mother suffered from cervical cancer （1.5%） were significantly lower than that in the control group （5.7%, all P 〈0.05）. The frequencies of HLA-DRB1 ＊ 14 in women with family history of cervical cancer （5.4%） and mother who suffered from cervical cancer （3.0%） were significantly lower than that in the control group （8.4%, all P 〈 0.05 ）. Conclusions There are similarity and difference in distribution of HLA-DRB1 allele polymorphisms between the Uyghur women with family history of cervical cancer from Hotan Moyu county and those from southern Xingjiang area. In general, the distribution of HLA-DRB1 allele polymorphism in women with family history of cervical cancer is similar to that reported in abroad. The results of this study support the role of susceptible and protective HLA gene detection in screening high risk persons for this cancer among Uyghur women from cervical cancer high risk areas in Xinjiang.
出处
《中华肿瘤杂志》
CAS
CSCD
北大核心
2012年第4期272-277,共6页
Chinese Journal of Oncology
基金
国家自然科学基金（30760222）
关键词
宫颈肿瘤
多态性
单核苷酸
人类白细胞抗原
维吾尔族
Uterine cervical neoplasms
Polymorphism, single nucleotide
Humanleukocyte antigen
Uyghur
