摘要
目的:对比研究地龙的鲜品和干品的可溶性蛋白及纤溶酶活性。方法:采用Bradfold方法测定蛋白含量,SDS-PAGE进行蛋白谱带的分析,并采用琼脂糖-纤维蛋白平板法进行纤溶酶活性的测定。结果:以干燥品计,鲜品的蛋白提取率为(13.89±0.86)%,蛋白含量为(63.14±0.38)%,体外纤溶酶活性为(8 743±17)IU·g-1;干品蛋白提取率为(9.82±0.75)%,蛋白含量为(45.16±0.12)%,体外纤溶酶活性为(206±14)IU·g-1;SDS-PAGE凝胶电泳结果显示,鲜品共显示12条蛋白谱带,干品的谱带较少,且不清晰。结论:鲜地龙的可溶性蛋白提取率、含量及体外纤溶酶活性均明显高于干品,且蛋白组分有较大的差异。
Objective：To study the dissolubility proteins and activity of fibrinolytic enzyme of fresh and dry earthworm comparably.Method： Bradfold method was used to detect protein content,SDS-PAGE was used to analyze the protein compositions,and the agarose-fibrin plate was used to detect the activity of fibrinolytic enzyme.Result： On dried basis,the protein extraction rate of fresh earthworm was（13.89±0.86）%,the protein content was（63.14±0.38）%,the activity of enzyme led to（8 743±17） IU·g-1;compared with dry earthworm,the protein extraction rate was（9.82±0.75）%,the protein content and enzyme activity were（45.16±0.12）% and（206±14） IU·g-1.The SDS-PAGE result showed that there were 12 protein bands in fresh earthworm and only several bands in dry earthworm.Conclusion： The extraction rate,content and enzyme activity of the dissolubility proteins at fresh earthworm were higher than dry earthworm,and the constituents exhibited obvious difference.
出处
《中国实验方剂学杂志》
CAS
北大核心
2012年第8期89-92,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
哈尔滨市科技创新人才研究专项资金项目(2011RFQYS080)
关键词
鲜地龙
干地龙
可溶性蛋白
纤溶酶活性
fresh earthworm
dry earthworm
dissolubility protein
activity of fibrinolytic enzyme
