摘要
目的:探索HPLC测定竭红跌打凝胶膏剂中阿魏酸的含量。方法:采用Kromasil C18色谱柱(4.6 mm×250 mm,5μm),以乙腈-水-三乙胺(9∶91∶0.15)用冰乙酸调节pH 4.0为流动相,流速1.0 mL·min-1,检测波长316 nm,柱温40℃。结果:阿魏酸在0.631～20.20 mg·L-1回归方程为A=0.794 8C+0.006 4(r=0.999 7),平均回收率为100.62%,RSD 3.08%(n=6)。结论:该方法操作简便,准确,重复性良好,可用于竭红跌打凝胶膏剂的质量控制。
Objective：To develop a HPLC method for determination off ferulic acid in Jiehong Dieda cataplasm.Method： Kromasil C18（4.6 mm×250 mm,5 μm） column was used,acetonitrile-water-triethylamine（9∶91∶0.15,pH was adjusted to 4.0 by acetic acid） as mobile phase.The flow rate was 1.0 mL·min-1,detection wavelength at 316 nm,and column temperature at 40 ℃.Result： The linear response ranges were 0.631-20.20 mg·L-1 with regression curve A=0.794 8C＋0.006 4（r=0.999 7） for ferulic acid.The average recovery of ferulic acid was 100.62%（RSD 3.08%,n=6）.Conclusion： A simple,accurate and reproducible HPLC method is provided for determination of ferulic acid in Jiehong Dieda cataplasm.
出处
《中国实验方剂学杂志》
CAS
北大核心
2012年第8期77-79,共3页
Chinese Journal of Experimental Traditional Medical Formulae
基金
广东省佛山市产学研专项资金项目(2007CT003)
