摘要
目的:探讨贯叶金丝桃中金丝桃素的最佳酶提取工艺。方法:以高效液相色谱法测定金丝桃素含量为指标,从纤维素酶、果胶酶、木聚糖酶、β-葡聚糖酶及复合酶SPE-007A中筛选出最优酶,采用单因素试验和正交试验优选提取工艺条件。结果:最优酶为纤维素酶,优选酶解工艺为酶用量2.0%,pH 4.8,温度50℃,酶解时间5 h,浸提倍数8倍。结论:该优选工艺高效、稳定。
Objective：To investigate optimum enzyme extraction technology of hypericin from Hypericum perforatum.Method： With the content of hypericin was determined by HPLC as index,optimum enzyme was selected from cellulase,pectinase,xylanase,β-glucanase and enzyme SPE-007A,extraction technology conditions was optimized by single factor test and orthogonal test.Result： The best enzyme was cellulase,optimum extraction process was as follows：the amount of enzyme 2.0%,pH 4.8,extraction temperature 50 ℃,hydrolysis time 5 h,liquid-solid ratio 8 times.Conclusion： This optimized technology was efficient and stable.
出处
《中国实验方剂学杂志》
CAS
北大核心
2012年第8期46-49,共4页
Chinese Journal of Experimental Traditional Medical Formulae
关键词
贯叶金丝桃
金丝桃素
酶解提取
Hypericum perforatum
hypericin
enzyme extraction
