摘要
目的通过检测乳腺原位癌及浸润性癌组织中NIMA相关蛋白激酶NEK2和细胞外信号调节激酶ERK2的表达,探讨其在乳腺癌变过程中发挥的作用。方法采用免疫组织化学ElivisionTMplus两步法,研究86例乳腺浸润性癌、10例乳腺原位癌、20例乳腺组织的表达情况。结果 NEK2在3组中的表达情况分别为87.2%、50.0%、10.0%。ERK2在3组中的表达情况分别为96.7%、80.0%、10.0%。NEK2与ERK2的表达呈正相关。NEK2蛋白和ERK2蛋白的表达均与患者发生肿瘤的淋巴结转移、病理学分级及临床分期呈明显正相关,而与患者的年龄,肿物大小,月经情况,组织分型无关。结论 ERK2相关信号传导通路和NEK2共同作用参与了乳腺癌的形成过程。
Objective To investgate the expression of NEK2 and ERK2 in the breast and breast carcinoma in situ and breast invasive carcinoma and to explore their role in the breast carcinogenesis. Methods The expression of NEK2 and ERK2 was studied in 86 cases of breast invasive carcinoma, 10 cases of breast invasive carcinoma and 20 cases of the breast with ElivisionTM plus two - step. Results The positive expression rate of NEK2 in the three tissues was 87.2%, 50. 0%, 10. 0%. The positive expression rate of ERK2 in the three tissues was 96.7%, 80.0%, 10. 0%. There was positive correlation in the expres- sion of NEK2 and ERK2 from the breast to the breast c^cinoma with cancerous changes. Negative relationship remained between the expression of NEK2, ERK2 and age, tumor size, Menstruasion status, histology type, and irrelevance with lymphatic me- tastasis and pothology stages, histologycal grade. Conclusion NEK2 and ERK2 signoling pathway is involved in the carcinogen- esis of the breast and perhaps they have interrelation interactively.
出处
《实用心脑肺血管病杂志》
2012年第4期649-651,共3页
Practical Journal of Cardiac Cerebral Pneumal and Vascular Disease
