摘要
对剑麻斑马纹病菌生长与产孢的最适培养基进行研究。结果表明:该病菌在燕麦培养基上生长速度最快,在蔗糖培养基上生长速度最慢,V8培养基最适生长;不同的培养基对产孢的作用有差异,所有培养基很少或没有产生孢子囊,而产生游动孢子的培养基,以PDA培养基最多;在培养基中加入不同的营养成分对产孢有较大影响,芝麻培养基+0.1%KNO3产生大量的孢子囊,其他很少或没有产生孢子囊,而PSA+0.1%KNO3产生游动孢子最多,10%剑麻汁培养基+0.1%KNO3产生的游动孢子最少。
The optimum culture medium of colony growth and sporulation for Phytophthora nicotianoe Breda was studied. Result showed that the pathogenic bacteria grew fastest on oatmeal glucose agar medium and slowest on potato sucrose agar medium,but V8 medium was the optimum culture medium in growth; Effect of different culture medium was different in sporulation：all culture medium producted sporangium few or no,and PDA medium producted zoospore most in all mediums;it had more effect to add different nutrient component in medium, it would product plenty of sporangium when sesame sucrose agar was added with 0.1%KNO3 and others do few or no,it would product zoospore most when 0.1%KNO3 was added in PSA medium,and it would product zoospore least when 0.1%KNO3 was added in 10% sisal extract medium
出处
《广东农业科学》
CAS
CSCD
北大核心
2012年第5期71-73,共3页
Guangdong Agricultural Sciences
基金
国家现代农业产业技术体系建设专项资金(CARS-19)
中央公益性科研院所中国热带农业科学院南亚热带作物研究所基本科研业务费专项基金(1251022011005)
关键词
剑麻斑马纹病菌
生长
产孢
Phytophthora nicotianae Breda
growth
sporulation
