摘要
目的 探讨 5 氨基酮戊酸光动力学疗法 (ALA PDT)体外伤杀肿瘤细胞的最佳照光时机和最佳照光能量。方法 用ALA诱导人喉癌细胞株HEP 2产生内源性的原卟啉Ⅸ ,以功率密度为 30mW/cm2 的He Ne激光 ,分别照射与ALA共同培养 1、4、8、12、48h的HEP 2细胞 ,并对与ALA共同培养 8h的HEP 2细胞 ,以同样功率密度的He Ne激光 ,分别照射 0 5、1 0、2 0、3 0、4 0min ,能量密度分别为 0 9、1 8、3 6、7 2、14 4J/cm2 。用MTT法测定照光后HEP 2细胞的死亡率。结果 (1)光照时机选择在ALA与HEP 2细胞培养 8h时杀伤效应最强 ;(2 )ALA PDT对HEP 2细胞的杀伤效应与照光能量呈正相关 (r=0 88,P <0 0 5 )。结论 ALA PDT能有效地杀伤体外培养的肿瘤细胞 ,其杀伤最佳效果与照光时机和照光能量有关。
Objective To study the optimal irradiation time and power density in photodynamic therapy with delta aminolevulinic acid(ALA PDT) for tumor cells photoinactivation in vitro. Methods Endogenous protoporthyrin Ⅸ (PPⅨ) was induced by ALA in HEP 2 cell line. The He Ne laser with a power density of 30 mW/cm 2 was used to irradiate the HEP 2 cells co cultured with ALA for 1, 4, 8, 12 and 48 h respectively. The HEP 2 cells co cultured with ALA for 8 h were harvested and were subjected to the irradiation with the same power density for 0 5, 1 0, 2 0, 3 0, 4 0 min respectively, and with the energy density being 0 9, 1 8, 3 6, 7 2 and 14 4 J/cm 2, respectively. The cell mortality following the irradiation was measured by using MTT assay.Results The strongest photoinactivation effect occured in the HEP 2 cells co cultured with 1 mmol/L ALA for 8 h. The killing activity of ALA PDT to the HEP 2 cells was positively correlated with the power density of the irradiation (r=0 88, P<0 05). Conclusions ALA PDT can effectively kill the cultured HEP 2 cells in vitro. The optimal killing activity was related to the time and the power density of the laser irradiation.
出处
《中国激光医学杂志》
CAS
CSCD
2000年第2期102-104,共3页
Chinese Journal of Laser Medicine & Surgery
关键词
氨基酮戊酸
光化学疗法
肿瘤细胞
喉癌
Aminolevulinic acid
Photochemotherapy
Tumor cells
cultured
